Abstract
Cells of Anacystis nidulans strain 1402-1 incorporate [methyl-3H]thymidine or [8-3H]adenine into DNA; in synchronous cultures (21/2 h full light, 1/2 h weak light, 5 h dark), this incorporation occurs in the dark to different extents according to the labeled precursor offered or to its specific activity. The specific activity of in vivo, uniformly labeled DNA decreases to half the initial value when the cells are grown in the absence of radioactive DNA precursors during the light phase; it does not decrease during the following dark phase. If unlabeled thymidine is given during the dark phase, the specific activity of the DNA starts to decrease at the onset of the next light phase. The time course of the decrease supports the hypothesis that all cells start their DNA replication immediately after illumination and that the first cells have completed if after 1.25 h. The slowest cells then need 3.75 h for completion of DNA replication. It is discussed whether the incorporation during the dark might be due to pool size effects.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bazarel, M., Helinski, D.R.: Circular DNA forms of colicinogenic factors E1, E2, and E3 from Escherichia coli. J. mol. Biol. 36, 185–194 (1968)
Chen, D., Osborne, D.J.: Ribosomal genes and DNA replication in germinating wheat embryos. Nature (Lond.) 225, 336–340 (1970)
Dashe, S.W., Howell, S.H.: Cautionary note on the use of [methyl-3H]thymidine to measure rates of chloroplast DNA synthesis in Chlamydomonas reinhardtii. J. Cell Biol. 69, 215–218 (1976)
Glaser, V.M., Al-Nuri, M.A., Groshev, V.V., Shestakov, S.V.: The labelling of nucleic acids by radioactive precursors in the blue-green algae Anacystis nidulans and Synechocystis aquatilis Sanv. Arch. Mikrobiol. 92, 217–226 (1973)
Herdman, M., Faulkner, B.M., Carr, N.G.: Synchronous growth and genome replication in the blue-green alga Anacystis nidulans. Arch. Mikrobiol. 73, 238–249 (1970)
Hurst, P.R., Gahan, P.B.: Turnover of DNA in ageing tissues of Lycopersicon esculentum. Ann. Bot. 39, 71–76 (1975)
Ingram, L.O., Fisher, W.D.: Selective inhibition of deoxyribonucleic acid synthesis by 2-deoxyadenosine in the blue-green bacterium Agmenellum quadruplicatum. J. Bacteriol. 112, 170–175 (1972)
Lorenzen, H., Kaushik, B.D.: Experiments with synchronous Anacystis nidulans. Ber. dtsch. Bot. Ges. 89, 491–498 (1976)
Pigott, G.H., Carr, N.G.: The assimilation of nucleic acid precursors by intact cells and protoplasts of the blue-green alga Anacystis nidulans. Arch. Mikrobiol. 79, 1–6 (1971)
Restaino, L., Frampton, E.W.: Labeling the deoxyribonucleic acid of Anacystis nidulans. J. Bacteriol. 124, 155–160 (1975)
Ssymank, V.: Influence of nitrogen deficiency on uridine incorporation into ribosomes in the green alga Chorella. Arch. Mikrobiol. 82, 311–324 (1972)
Ssymank, V.: Incorporation of different labeled precursors into chloroplast RNA of Chlorella. Planta (Berl.) 111, 157–166 (1973)
Ssymank, V., Steup, M., Senger, H.: Studies on nucleic acids in plastids of the pigment mutant C-2A' of Scenedesmus obliquus. Plant Cell Physiol. 17, 787–798 (1976)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ssymank, V., Kaushik, B.D. & Lorenzen, H. Synthesis of DNA during the cell cycle of synchronous Anacystis nidulans . Planta 135, 13–17 (1977). https://doi.org/10.1007/BF00387969
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00387969