Abstract
Gibberellic acid (GA3) stimulates water uptake in castor beans and increases the activity of certain enzymes associated with lipid mobilisation.
The effect of the GA3 on the enzymes is possibly due to a general effect of the growth substance on protein synthesis. Gibberellic acid advanced the appearance of rRNA and poly (A+)RNA in castor bean endosperms without specifically stimulating the synthesis of particular mRNA species. Thus these increased levels of mRNA and rRNA may act synergistically to affect the rate of a predetermined pattern of protein synthesis.
Similar content being viewed by others
Abbreviations
- SDS:
-
sodium dodecyl sulphate
- GA3 :
-
gibberellic acid
- PAGE:
-
polyacrylamide gel electrophoresis
References
Allfrey, J.M., Northcote, D.H. (1977) The effects of the axis and plant hormones on the mobilisation of storage materials in the ground-nut (Arachis hypogaea) during germination. New Phytol.78, 547–563
Beevers, H. (1969) Glyoxysomes of castor bean endosperm and their relation to gluconeogenesis. Ann. N.Y. Acad. Sci.168, 313–324
Ben Tal, Y., Varner, J.E. (1974) An early response to gibberellic acid not requiring protein synthesis. Plant Physiol.54, 813–816
Bilderback, D.E. (1974) The regulatory role of the embryo on the development of isocitrate lyase activity during germination of Ponderosa pine seeds. Physiol. Plant.31, 200–203
Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Anal. Biochem.72, 248–252
Cheung, C.P., Wu, J., Suhodolnik, R.J. (1979) Dependence of protein synthesis on RNA synthesis during the early hours of germination of wheat embryos. Nature (London)277, 66–67
Choinski, J.S. (1981) Gibberellins and the development of glyoxalate cycle enzyme activity in castor bean endosperm. Plant Physiol.67 (suppl.), 149
Chrispeels, M.J., Varner, J.E. (1967) Gibberellic acid-enhanced synthesis and release of α-amylase and ribonuclease by isolated barley aleurone layers. Plant Physiol.42, 398–406
Doig, R.I., Colbourne, A.J., Morris, G., Laidman, D.L. (1975) The induction of glyoxysomal enzyme activities in the aleurone cells of germinating wheat. J. Exp. Bot.26, 387–398
Evins, W.H., Varner, J.E. (1971) Hormone-controlled synthesis of endoplasmic reticulum in barley aleurone cells. Proc. Natl. Acad. Sci. USA68, 1631–1633
Gonzalez, E. (1978) Effect of gibberellin A3 on the endoplasmic reticulum and on the formation of glyoxysomes in the endosperm of germinating castor bean. Plant Physiol.62, 449–453
Hawker, J.S., Bungay, D.W. (1976) Isocitrate lyase in germinating seeds ofPrunus dulcis. Phytochemistry15, 79–81
Jacobsen, J.V., Higgins, T.J.V., Zwar, J.A. (1979) In: The plant seed: development, preservation and germination, pp. 241–262, Rubenstein, I., Phillips, R.L., Green, C.E., Gengenbach, B.G., eds. Academic Press, New York
Johnson, K.D., Kende, H. (1971) Hormonal control of lecithin synthesis in barley aleurone cells: regulation of the CDP-choline pathway by gibberellin. Proc. Natl. Acad. Sci. USA68, 2674–2677
Khan, A.A. (1977) In: The physiology and biochemistry of seed dormancy and germination, Khan, A.A., ed. North Holland Publishing Co., Amsterdam
Koehler, D.E., Varner, J.E. (1973) Hormonal control of orthophosphate incorporation into phospholipids of barley aleurone layers. Plant Physiol.52, 208–214
Laemmli, U.K. (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature (London)227, 680–684
Laskey, R.A., Mills, A.D. (1975) Quantitative film detection of3H and14C in polyacrylamide gels by fluorography. Eur. J. Biochem.56, 355–341
Marriott, K.M., Northcote, D.H. (1975a) The breakdown of lipid reserves in the endosperm of germinating castor beans. Biochem. J.148, 139–144
Marriott, K.M., Northcote, D.H. (1975b) The induction of enzyme activity in the endosperm of germinating castor bean seeds. Biochem. J.152, 65–70
Martin, C.R., Northcote, D.H. (1981) Qualitative and quantitative changes in mRNA of castor beans during the initial stages of germination. Planta151, 189–197
Martin, C.R., Northcote, D.H. (1982) The action of exogenous gibberellic acid on isocitrate lyase mRNA in germinating castor bean seeds. Planta154, 174–183
Mozer, T.J. (1980) Control of protein synthesis in barley aleurone layers by the plant hormones, gibberellic acid and abscisic acid. Cell20, 479–485
Pinfield, N.J. (1968) The promotion of isocitrate lyase activity in hazel cotyledons by exogenous gibberellin. Planta82, 337–341
Roberts, L.M., Lord, J.M. (1979) Ribonucleic acid synthesis in germinating castor bean endosperm. J. Exp. Bot.30, 739–749
Schröder, J., Kreuzaler, F., Schäfer, E., Hahlbrock, K. (1979) Concomitant induction of phenylalanine ammonia-lyase and flavone synthase mRNas in irradiated plant cells. J. Biol. Chem.254, 57–65
Weir, E.M., Riezman, H., Grienenberger, J.-M., Becker, W.M., Leaver, C.J. (1980) Regulation of glyoxysomal enzymes during germination of cucumber. Eur. J. Biochem.112, 469–477
Wrigley, A., Lord, J.M. (1977) The effects of gibberellic acid on organelle biogenesis in the endosperm of germinating castor bean seeds. J. Exp. Bot.28, 345–353
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Martin, C., Northcote, D.H. The action of exogenous gibberellic acid on protein and mRNA in germinating castor bean seeds. Planta 154, 168–173 (1982). https://doi.org/10.1007/BF00387912
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00387912