Abstract
The results reported in this paper provide strong evidence to support the belief that the small percentage of phytochrome recovered in low-speed centrifugation pellets, when prepared in the absence of divalent cations after various in vivo irradiations, is not simply a manifestation of non-specific co-precipitation of soluble phytochrome.
The far-red reversibility of the observed near-doubling of phytochrome pelletability after in vivo red irradiation indicates that phytochrome pelletability in the absence of divalent cations is a phytochrome-controlled response. The characteristics of the pelleted phytochrome indicate a strong, hydrophobic interaction with membranes. A tentative proposal to explain the observed characteristics of the association of phytochrome with membranous material in the absence of divalent cations after different in vivo irradiations has been put forward.
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Abbreviations
- Pfr:
-
phytochrome in the far-red light absorbing form
- Pr:
-
phytochrome in the fat-red light absorbing form
- Ptot:
-
total phytochrome
- R:
-
red light irradiation
- FR:
-
far-red light irradiation
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Watson, P.J., Smith, H. Integral association of phytochrome with a membranous fraction from etiolatedAvena shoots: red/far-red photoreversibility and in vitro characterization. Planta 154, 121–127 (1982). https://doi.org/10.1007/BF00387904
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DOI: https://doi.org/10.1007/BF00387904