Abstract
The halotolerant phytoplankton alga Dunaliella tertiolecta was found to be able to synthesize considerable amounts of sucrose. Because this is in contradiction to the current literature, the physiologic conditions required for sucrose biosynthesis were investigated. Sucrose is only synthesized above a jump temperature, which depends on the NaCl concentration in the medium. Unlike earlier results, which showed that glycerol is synthesized only under photosynthetic conditions, sucrose is also produced in the dark, however at a reduced rate. In the dark, sucrose synthesis ceases after 24 h, thus indicating that sucrose is formed from storage substances.
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Ben-Amotz, A.: Adaptation of the unicellular alga Dunaliella parva to a saline environment. J. Phycol. 11, 50–54 (1975)
Bird, I.F., Cornelius, M.J., Keys, J., Whittingham, C.P.: Intracellular site of sucrose synthesis in leaves. Phytochemistry 13, 59–64 (1974)
Craigie, J.S., McLachlan, J.: Glycerol as a photosynthetic product in Dunaliella tertiolecta Butcher. Can. J. Bot. 42, 777–778 (1964)
Craigie, J.S., McLachlan, J., Ackman, R.G., Tocher, C.S.: Photosynthesis in algae. III. Distribution of soluble carbohydrates and dimethyl-β-propiothetin in marine unicellular Chlorophyceae and Prasinophyceae. Can. J. Bot. 45, 1327–1334 (1967)
Craigie, J.S., McLachlan, J., Majak, W., Ackman, R.G., Tocher, C.S.: Photosynthesis in algae. II. Green algae with special reference to Dunaliella spp. and Tetraselmis spp. Can. J. Bot. 44, 1247–1254 (1966)
Frank, G., Wegmann, K.: Physiology and biochemistry of glycerol biosynthesis in Dunaliella. Biol. Zbl. 93, 707–723 (1974)
Heber, U., Willenbrink, J.: Sites of synthesis and transport of photosynthetic products within the leaf cell. Biochim. Biophys. Acta 82, 313–324 (1964)
Heldt, H.W., Sauer, F.: The inner membrane of the chloroplast envelop as the site of specific metabolic transport. Biochim. Biophys. Acta 234, 83–91 (1971)
Hinton, R.H., Burge, M.L.E., Hartman, G.C.: Sucrose interference in the assay of enzymes and protein. Anal. Biochem. 29, 248–256 (1969)
Kelly, G.J., Latzko, E., Gibbs, M.: Regulatory aspects of photosynthetic metabolism. Ann. Rev. Plant Physiol. 27, 181–205 (1976)
Latzko, E., Zimmermann, G., Feller, U.: Evidence for a hexosediphosphatase from the cytoplasm of spinach leaves. Hoppe Seyler's Z. Physiol. Chem. 355, 321–326 (1974)
Metzner, H., Rau, H.-J., Senger, H.: Untersuchungen zur Synchronisierbarkeit einzelner Pigmentmangelmutanten von Chlorella. Planta 65, 186–194 (1965)
Schürmann, P.: Separation of phosphate esters and algal extracts by thin layer electrophoresis and chromatography. J. Chromatogr. 39, 507–509 (1969)
Stewart, W.D.P.: Algal physiology and biochemistry. Oxford: Blackwell 1974
Wegmann, K.: Der Weg des Kohlenstoffs bei der Photosynthese und Dunkelfixierung in Dunaliella spec. Thesis Universität Tübingen (W-Germany) (1968)
Wegmann, K.: On the pathways of CO2 fixation in Dunaliella. In: Progress in photosynthesis research, pp. 1559–1564) Vol. III, Metzner, H., ed. 1969
Wegmann, K.: Osmotic regulation of photosynthetic glycerol production in Dunaliella. Biochim. Biophys. Acta 234, 317–323 (1971)
Winkenbach, F., Grant, B.R., Bidwell, R.G.S.: The effects of nitrate, nitrite, and ammonia on photosynthetic carbon metabolism of Acetabularia chloroplast preparations compared with spinach chloroplasts and whole cells of Acetabularia and Dunaliella. Can. J. Bot. 50, 2545–2551 (1972)
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Müller, W., Wegmann, K. Sucrose biosynthesis in Dunaliella . Planta 141, 155–158 (1978). https://doi.org/10.1007/BF00387882
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DOI: https://doi.org/10.1007/BF00387882