Abstract
Chloroplasts prepared from lysed protoplasts of barley mesophyll contain 2–8% of the total acid proteinase activity. This residual activity is not associated with intact chloroplasts isolated by means of density gradient centrifugation. Vacuoles isolated from lysed protoplasts contain 80–85% of the total acid proteinase activity, indicating that the enzyme(s) which is presumably responsible for the degradation of chloroplastic proteins is located largely in the central vacuoles of mesophyll cells.
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Heck, U., Martinoia, E. & Matile, P. Subcellular localization of acid proteinase in barley mesophyll protoplasts. Planta 151, 198–200 (1981). https://doi.org/10.1007/BF00387823
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DOI: https://doi.org/10.1007/BF00387823