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Qualitative and quantitative changes in mRNA of castor beans during the initial stages of germination

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Abstract

The increase in extractable RNA during the initial germination stages of castor bean was measured, for both the embryonic axis and the storage endsperm. The extractable rRNA increased between 24 and 72 h after initial imbibition in the embryo and 48–72 h in the endosperm. The mRNA species present over the first 6 days of germination were identified by the products from in vitro translation. The mRNA from dry seeds gave predominantly low molecular weight polypeptide products. Between 0.5 and 1 h of initial imbibition new mRNA species were detectable and the qualitative changes were largely complete by 8 h, some 16 h–40 h before the detectable quantitative changes. Despite the large variations in enzyme activity occurring 48 h–192 h after imbibition, the mRNA species qualitatively varied very little, after this initial change, up to 144 h after imbibition. In the light of this large, early, qualitative change in mRNA, the possible importance of long-lived mRNA in seed germination is discussed.

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Abbreviations

SDS:

sodium dodecyl sulphate

TMV:

tobacco mosaic virus

DEP:

diethyl pyrocarbonate

MDL:

message-dependent reticulocyte lysate

TCA:

trichloroacetic acid

MW:

molecular weight

PAGE:

polyacrylamide gel electrophoresis

References

  • Beachy, R.N., Barton, K.A., Thomson, J.F., Madison, J.T. (1980) In vitro synthesis of the α and α′ subunits of the 7s storage proteins (conglycinin) of soybean seeds. Plant Physiol. 65, 990–994

    Google Scholar 

  • Bewley, J.D., Black, M. (1978) Physiology and biochemistry of seeds, 1. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Bowden, L., Lord, J.M. (1976) The cellular origin of glyoxysomal proteins in germinating castor bean endosperm. Biochem. J. 154, 501–506

    PubMed  Google Scholar 

  • Bowden-Bonnett, L., Lord, J.M. (1979) Isolation and cell-free translation of total messenger RNA from germinating castor bean endosperm. Plant Physiol. 63, 769–773

    Google Scholar 

  • Brocklehurst, P.A., Fraser, R.S.S. (1980) Ribosomal RNA integrity and rate of seed germination. Planta 148, 417–421

    Google Scholar 

  • Caers, L.I., Peumans, W.J., Carlier, A.R. (1979) Preformed and newly synthesised messenger RNA in germinating wheat embryos. Planta 144, 491–496

    Google Scholar 

  • Delseny, M., Aspart, L., Guitton, Y. (1977) The dissappearance of stored polyadenylic acid and mRNA during early germination of radish (Raphanus sativus L.) embryo axes. Planta 135, 125–128

    Google Scholar 

  • Dobrzanska, M., Tomaszewski, M., Grzelczak, Z., Rejman E., Buchowicz, J. (1973) Cascade activation of genome transcription in wheat. Nature (London) 244, 507–509

    Google Scholar 

  • Dudley, K., Northcote, D.H. (1978) Comparison of the in vitro translation products of mRNA isolated from suspension cultures of Phaseolus vulgaris grown on maintainance and induction medium. Planta 138, 41–48

    Google Scholar 

  • Gerhardt, B.P., Beevers, H. (1970) Developmental studies on glyoxysomes in Ricinus endosperm. J. Cell Biol. 44, 94–102

    Article  Google Scholar 

  • Gordon, M.E., Payne, P.I. (1977) In vitro translation of poly A+ rich RNA from dry and imbibing seeds of rape. In: Translation of natural and synthetic polynucleotides, pp. 228–231, Legocki, A.B. ed., Poznan Agricultural University, Poznan

    Google Scholar 

  • Gray, R.E., Cashmore, A.R. (1976) RNA synthesis in plant leaf tissue: The characterisation of messenger RNA species lacking and containing polyadenylic acid. J. Mol. Biol. 108, 595–608

    PubMed  Google Scholar 

  • Hammett, J.R., Katterman, F.R. (1975) Storage and metabolism of poly (adenylic acid) mRNA in germinating cotton seeds. Biochemistry 14, 4375–4379

    PubMed  Google Scholar 

  • Harris, B., Dure, L. (1978) Developmental regulation in cotton seed germination; polyadenylation of stored messenger RNA. Biochemistry 17, 3250–3256

    PubMed  Google Scholar 

  • Higgins, T.J.V., Zwar, J.A., Jacobsen, J.V. (1976) Gibberellic acid enhances the level of translatable mRNA for the α-amylase in barley aleurone layers. Nature (London) 260, 166–169

    Google Scholar 

  • Ihle, J.N., Dure, L. (1969) Synthesis of a protease in germinating cotton cotyledons catalysed by mRNA synthesised during embryogenesis. Biochem. Biophys. Res. Commun. 36, 705–710

    PubMed  Google Scholar 

  • Ihle, J.N., Dure, L. (1970) Hormonal regulation of translation inhibition requiring RNA synthesis. Biochem. Biophys. Res. Commun. 38, 995–1007

    PubMed  Google Scholar 

  • Ihle, J.N., Dure, L. (1972) The developmental biochemistry of cotton seed embryogenesis and germination. J. Biol. Chem. 247, 5048–5055

    PubMed  Google Scholar 

  • Kaufmann, Y., Milcarek, C., Berissi, H., Penman, S. (1977) Hela cell poly A- mRNA codes for a subset of poly A+ mRNA-directed proteins with an actin as a major product. Proc. Natl. Acad. Sci. USA 74, 4801–4805

    PubMed  Google Scholar 

  • Laemli, U.K. (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature (London) 227, 680–684

    Google Scholar 

  • Laskey, R.A., Mills, A.D. (1975) Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur. J. Biochem. 56, 335–341

    PubMed  Google Scholar 

  • Marcus, A., Feeley, J., Volcani, T. (1966) Protein synthesis in imbibed seeds III. Kinetics of amino acid incorporation, ribosome activation and polysome formation. Plant Physiol. 41, 1167–1172

    Google Scholar 

  • Marriot, K.M., Northcote, D.H. (1975) Induction of enzyme activity in the endosperm of germinating castor bean seeds. Biochem. J. 152, 65–70

    PubMed  Google Scholar 

  • Marriot, K.M., Northcote, D.H. (1977) The influence of abscisic acid, 3′, 5′ cyclic phosphate and gibberellic acid on the induction of isocitrate lyase activity in the endosperm of germinating castor bean seeds. J. Exp. Bot. 28, 219–224

    Google Scholar 

  • Merercandani, N., Naylor, J.M. (1972) Onset of the synthesis of RNA and protein in mature aleurone tissue of wid oats (Avena fatua). Can. J. Bot. 50, 305–313

    Google Scholar 

  • Mozer, T.J. (1980) Control of protein synthesis in barley aleurone layers by the plant hormones gibberellic acid and abscisic acid. Cell 20, 479–485

    PubMed  Google Scholar 

  • Payne, P.I. (1976) The long-lived messenger ribonucleic acid of flowering-plant seeds. Biol. Rev. 51, 329–363

    Google Scholar 

  • Payne, P.I., Gordon, M.E., Dobrzanska, M., Parker, M.L., Barlow, P.W. (1977) The long-lived messenger RNA of dry seeds: Evidence for its existance, intracellular location and role in germination. Coll. Int. CNRS 261, 487–499

    Google Scholar 

  • Pelham, H.R.B., Jackson, R.J. (1976) An efficient mRNA-dependent translation system from reticulocyte lysates. Eur. J. Biochem. 67, 247–256

    PubMed  Google Scholar 

  • Radin, J.W., Trelease, R.N. (1976) Control of enzyme activities in cotton cotyledons during maturation and germination. Plant Physiol. 57, 902–905

    Google Scholar 

  • Ragg, H., Hahlbrock, K. (1980) Messenger RNA coding for phenylalanine ammonia lyase. Eur. J. Biochem. 103, 323–330

    PubMed  Google Scholar 

  • Roberts, L.M., Lord, J.M. (1979a) Ribonucleic acid synthesis in germinating castor bean endosperm. J. Exp. Bot. 30, 739–749

    Google Scholar 

  • Roberts, L.M., Lord, J.M. (1979b) Developmental changes in the activity of messenger RNA isolated from germinating castor bean endosperm. Plant Physiol. 64, 630–634

    Google Scholar 

  • Smith, R.H., Schubert, A.M., Benedict, C.R. (1974) The development of isocitrate lyase activity in germinating cotton seed. Plant Physiol. 54, 197–200

    Google Scholar 

  • Spiegal, S., Marcus, M. (1975) Polyribosome formation in early wheat embryo germination independent of either transcription or polyadenylation. Nature (London) 256, 228–230

    Google Scholar 

  • Taneja, S.R., Sachar, R.C. (1976) Enzyme synthesis by conserved messages in germinating wheat embryos. Phytochemistry 15, 1589–1594

    Google Scholar 

  • Walbot, V., Capdevila, A., Dure, L. (1974) Action of 3′d adenosine (cordycepin) and 3′d cytidine on the translation of the stored mRNA of cotton cotyledons. Biochem. Biophys. Res. Commun. 60, 103–110

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, University of Cambridge, CB2 1QW, Cambridge, UK

    Cathie Martin & D. H. Northcote

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  1. Cathie Martin
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  2. D. H. Northcote
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Martin, C., Northcote, D.H. Qualitative and quantitative changes in mRNA of castor beans during the initial stages of germination. Planta 151, 189–197 (1981). https://doi.org/10.1007/BF00387822

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  • DOI: https://doi.org/10.1007/BF00387822

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