Summary
We report the nucleotide sequence of three tRNA genes from maize mitochondria. The genes are located in two BamHI fragments, 3.55 and 5.7 kb long, adjacent to the S2 sequence in the maize mitochondrial genome. On the 3.55 kb BamHI fragment, we have characterized a tRNACys (GCA) gene. A strong sequence homology of this tRNACys (GCA) gene with its chloroplast counterpart in wheat suggests that it may be part of a chloroplast DNA insertion into the mitochondrial genome. This gene has been found to be transcribed in the mitochondrion. Two tRNA genes are located on the 5.7 kb BamHI fragment, separated from each other by 250 bp. One is a mitochondrial tRNASer (GCU) gene. The other, a non-transcribed tRNAPhe-like gene, is interrupted by a 49 base-pair inserted DNA sequence in the variable loop and has a Leu (UAA) anticodon.
Similar content being viewed by others
References
Anderson S (1981) Nucleic Acids Res 9:3015–3027
Birnboim HC, Doly J (1979) Nucleic Acids Res 7:1513–1523
Carlson JE, Brown GL, Kemble RJ (1986a) Curr Genet 11:151–160
Carlson JE, Brown GL, Kemble RJ (1986b) Curr Genet 11:161–163
Chisholm GE, Genbauffe FS, Cooper TG (1984) Proc Natl Acad Sci USA 81:2965–2969
Dale RMK, Mendu N, Ginsburg H, Kridl JC, (1984) Plasmid 11:141–150
Deno H, Sugiura M (1984) Proc Natl Acad Sci USA 81:405–408
Dewey RE, Levings CS III, Timothy DH (1986) Cell 44:439–449
Dron M, Hartmann C, Rode A, Sevignac M (1985) Nucleic Acids Res 13:8603–8610
Fox DT (1983) Nature 301:371–372
Gauss DH, Sprinzl M (1985) Nucleic Acids Res (Suppl) 13:r1-r131
Guillemaut P, Burkard G, Weil JH (1972) Phytochem 11:2217–2220
Houchins JP, Ginsburgh H, Rohrbaugh M, Dale RMK, Schardl CL, Hodge TP, Lonsdale DM (1986) EMBO J 5:2781–2788
Iams KP, Heckmann JE, Sinclair JH (1985) Plant Mol Biol 4:225–232
Ishikura H, Yamada Y, Nishimura S (1971) FEBS Lett 16:69–70
Koch W, Edwards K, Kössel H (1981) Cell 25:203–213
Levings CS III, Sederoff RR (1983) Proc Natl Acad Sci USA 80:4055–4059
Lonsdale DM, Thompson RD, Hodge TP (1981) Nucleic Acids Res 9:3657–3669
Lonsdale DM, Hodge CJ, Howe CJ, Stern DB (1983) Cell 34:1007–1014
Lonsdale DM (1984) Movement of genetic material between chloroplast and mitochondria in higher plants. In: Hohn B, Dennis L (eds) Advances in plant research, vol 2. Springer, Wien New York, pp 51–60
Lonsdale DM, Hodge TP, Fauron CMR (1984) Nucleic Acids Res 12:9249–9261
Marechal L, Guillemaut P, Grienenberger JM, Jeannin G, Weil JH (1985) FEBS Lett 184:289–293
Marechal L, Guillemaut P, Grienenberger JM, Jeannin G, Weil JH (1986) Plant Mol Biol 7:245–253
Marechal L, Runeberg-Roos P, Grienenberger JM, Colin J, Weil JH, Lejeune B, Quetier F, Lonsdale DM (1987) Curr Genet 12:91–98
Moon E, Koa TH, Wu R (1987) Nucleic Acids Res 15:611–630
Parks TD, Dougherty WG, Levings CS III, Timothy DH (1984) Plant Physiol 76:1079–1082
Quigley F, Grienenberger JM, Weil JH (1985) Plant Mol Biol 4:305–310
Quigley F, Weil JH (1985) Curr Genet 9:495–503
Rigby PW, Dieckmann M, Rhodes C, Berg P (1977) J Mol Biol 113:237–251
Russell D, Muskavitch KMT, Bogorad L (1986) Nucleic Acids Res 15:370
Ruther U (1980) Mol Gen Genet 179:475–477
Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Schwarz Z, Kössel H (1980) Nature 283:739–742
Sharp SJ, Schaack J, Cooley L, Burke DJ, Sool D (1985) CRC Critical Rev Biochem 19:107–144
Silberklang M, Gillum AM, Rajbhandary UL (1979) Methods Enzymol 59:58–109
Southern E (1975) J Mol Biol 98:503–518
Sprinzl M, Gauss DH (1984) Nucleic Acids Res (Suppl) 12:r59-r131
Stern DB, Lonsdale DM (1982) Nature 299:699–702
Stern DB, Palmer JD (1984) Proc Natl Acad Sci USA 81:1946–1950
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wintz, H., Grienenberger, JM., Weil, JH. et al. Location and nucleotide sequence of two tRNA genes and a tRNA pseudo-gene in the maize mitochondrial genome: evidence for the transcription of a chloroplast gene in mitochondria. Curr Genet 13, 247–254 (1988). https://doi.org/10.1007/BF00387771
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00387771