, Volume 107, Issue 2, pp 131–144 | Cite as

Fine structure of swarmers of Cladophora and Chaetomorpha

III. Wall synthesis and development
  • David G. Robinson
  • R. K. White
  • R. D. Preston


Naked swarmers of Cladophora have been collected and wall synthesis and development have been followed using the techniques of freeze-etching and sectioning. Swarmers frozen after 9 hours liberation have lost their flagella, developed the characteristic fibrous layer and show the initial stages of wall production. Both the first formed (randomly oriented) and the later (more ordered) microfibrils appear to have a distinct granular texture. Occasionally linear arrays of granules up to 4 μm long may be seen. After 5 days settling a thick wall composed of almost transversely oriented microfibrils is present and a rhizoid is pushed out. Also characteristic of this stage is the central localisation of cell components and peripheral vacuolar distribution. Longitudinally oriented microtubules also reappear at this stage having been absent during carlier wall formation.

A possible relationship between the cortical microtubules of the motile swarmer and the development of the fibrous layer is suggested.


Fine Structure Central Localisation Linear Array Cell Component Thick Wall 
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  1. Baker, J. R. J.: Stuies on the ship-fouling alga Ectocarpus. Ph. D. Thesis. Univ. of Leeds (1971).Google Scholar
  2. Barnett, J. R., Preston, R. D.: Arrays of granules associated with the plasmalemma in swarmers of Cladophora. Ann. Bot., N. S. 34, 1011–1017 (1970).Google Scholar
  3. Bouck, G. B.: Extracellular microtubules. The origin, structure and attachment of flagellar hairs in Fucus and Ascophyllum antherozoids. J. Cell Biol. 40, 446–460 (1969).CrossRefPubMedGoogle Scholar
  4. Caulfield, D. F.: Crystallite sizes in wet and dry Valonia ventricosa. Text. Res. J. 41, 267–274 (1971).Google Scholar
  5. Evans, L. V., Christie, A. O.: Studies on the ship-fouling alga Enteromorpha. I. Aspects of the fine structure and biochemistry of swimming and newly-settled zoospores. Ann. Bot., N.S. 34, 451–466 (1970).Google Scholar
  6. Hengstenberg, J., Mark, H.: Über Form und Größe der Micelle von Cellulose und Kautschuk. Z. Kryst. 69, 271–286 (1928).Google Scholar
  7. Marx-Figini, M.: Investigations on biosynthesis of cellulose. DPw and yield of cellulose of the alga Valonia in the presence of colchicine. Biochim. biophys. Acta (Amst.) 237, 75–77 (1971).Google Scholar
  8. Nicolai, E.: Wall deposition in Chaetomorpha melagonium (Cladophorales). Nature (Lond.) 180, 491–493 (1975).Google Scholar
  9. Nicolai, E., Preston, R. D.: Cell wall studies in the Chlorophyceae. III. Differences in structure and development in the Cladophoraceae. Proc. roy. Soc. B 151, 244–255 (1959).Google Scholar
  10. Nieduszynski, I., Preston, R. D.: Crystallite size in natural cellulose. Nature (Lond.) 225, 273–274 (1970).Google Scholar
  11. Ohad, I., Mejzler, D.: On the ultrastructure of cellulose microfibrils. J. Polymer Sci. A 3, 399–409 (1965).Google Scholar
  12. Preston, R. D.: Structural and mechanical aspects of plant cell walls with particular reference to synthesis and growth. In: Formation of wood in forest trees, p. 169–188 (M. H. Zimmermann, editor). London-New York: Acad. Press 1964.Google Scholar
  13. Preston, R. D., Goodman, R. N.: Structural aspects of cellulose microfibrils biosynthesis. J. roy. micr. Soc. 88, 513–527 (1968).Google Scholar
  14. Robinson, D. G.: Fine structure of swarmers of Cladophora and Chaetomorpha. II. The Chloroplast. J. Cell Sci. 10, 307–314 (1972).PubMedGoogle Scholar
  15. Robinson, D. G., Preston, R. D.: Fine structure of swarmers of Cladophora and Chaetomorpha. I. The plasmalemma and golgi apparatus in naked swarmers. J. Cell Sci. 9, 581–601 (1971a).PubMedGoogle Scholar
  16. Robinson, D. G., Preston, R. D.: Studies on the fine structure of Glaucocystis nostochinearum Itzigs. I. Wall structure. J. exp. Bot. 22, 635–643 (1971b).Google Scholar
  17. Robinson, D. G., Preston, R. D.: Studies on the fine structure of Glaucocystis nostochinearum Itzigs. II. Membrane morphology and taxonomy. Br. Phycol. J. 6, 113–128 (1971c).Google Scholar
  18. Robinson, D. G., Preston, R. D.: Plasmalemma structure in relation to microfibril biosynthesis in Oocystis. Planta (Berl.) 104, 234–246 (1972).Google Scholar
  19. Spurr, A. R.: A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26, 31–43 (1969).PubMedGoogle Scholar
  20. Stephens, R. E.: Reassociation of microtubule protein. J. mol. Biol. 33, 517–519 (1968).PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • David G. Robinson
    • 1
  • R. K. White
    • 1
  • R. D. Preston
    • 1
  1. 1.Astbury Dept. of BiophysicsUniversity of LeedsLeedsUK

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