Summary
Isolated leaves, leaf fragments and pieces of the midrib portion devoid of lamina, of Heloniopsis orientalis were grown on an inorganic nutrient medium without organic nutrients and growth regulators in order to investigate their regenerative ability. Bud formation in intact, attached leaves occurs only at the tip, in isolated leaves at the tip and the base, whereas leaf fragments cut transversely at a distance from the tip and isolated midrib pieces form numerous shoot buds in a random distribution. Lamina fragments lacking midrib frequently fail to regenerate even after a long time of culture. It is suggested that endogeneous growth regulators in the leaf, especially the vascular tissues, play an important role in bud initiation. Very young leaves of Heloniopsis are capable forming buds and roots when isolated from the mother plants.
Similar content being viewed by others
References
Broertjes, C., Haccius, B., Weidlich, S.: Adventitious bud formation on isolated leaves and its significance for mutation breeding. Euphytica 17, 321–344 (1968).
Caruso, J. L.: Bud formation in excised stem segments of Verbascum thapsus. Amer. J. Bot. 58, 429–431 (1971).
Chlayah, A., Tran Thanh Van, M.: Comparison of the localization of nucleic acid synthesis during bud formation on leaf fragment and on intact undetached leaves of Begonia rex Putz. Biol. Plant. (Praha) 13, 184–188 (1971).
Churchill, N. E., Arditti, J., Ball, E. A.: Clonal propagation of orchids from leaf tips. Amer. Orch. Soc. Bull. 42, 109–113 (1971).
Cutter, E. G.: Regeneration in Zamioculcas: an experimental study. Ann. Bot. 26, 55–70 (1962).
Kumazawa, M.: Plantlet formation on the leaf tip of Heloniopsis orientalis (Liliac.): A histogenetic and experimental study (Jap. with Engl. summary), J. Geobot. 8, 34–39 (1960).
Skoog, F., Miller, C. O.: Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symp. Soc. exp. Biol. 11, 118–131 (1957).
Tran Thanh Van, M., Drira, A.: Definition of a simple experimental system of directed organogenesis de novo: organ neoformation from epidermal tissue of Nautilocalyx lynchei. Colloq. Internat. C.N.R.S. (Cult. Tissues Plant.) 193, 169–176 (1971).
Vardar, Y., Acarer, P.: Auxin in relation to the development of epiphyllous buds in Bryophyllum. Øyton 8, 109–118 (1957).
Zenkteler, M. A.: Development of new plants from leaves and roots of Atropa belladonna L. in the in vitro culture. Acta Soc. Bot. Polon. 60, 305–315.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kato, Y., Kawahara, S. Bud formation in leaves, leaf fragments and midrib pieces of Heloniopsis orientalis (Liliaceae) . Planta 107, 111–120 (1972). https://doi.org/10.1007/BF00387717
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00387717