Skip to main content
SpringerLink
Log in

Expression of Annexin I in freshly isolated human epidermal cells and in cultured keratinocytes

  • Original Contributions
  • Published:
Archives of Dermatological Research Aims and scope Submit manuscript

Abstract

Annexin I belongs to a newly characterized family of intracellular proteins involved in the regulation of the production of inflammatory lipid mediators such as prostaglandins and leucotrienes. Annexin I (named p35, lipocortin I or calpactin II) was initially described as a protein inducible by glucocorticoids. In the skin, the role of annexins has still not been elucidated. In the study reported here we investigated the expression of annexin I both in freshly isolated epidermal cells and in cultured keratinocytes using immunofluorescence, FACS analysis and immunoblotting techniques. Using epidermal cells freshly isolated from normal skin, annexin I was detected by double immunostaining mainly in basal and suprabasal keratinocytes. Langerhans cells isolated from Ficoll gradient were faintly stained compared with keratinocytes. Annexin I was also highly expressed in keratinocytes maintained in culture in a serum-free medium without hydrocortisone. By confocal microscopy, annexin I was shown to be mainly localized in the cytoplasm of the cells. The protein was characterized by Western blot and immunoprecipitation as a 35-kDa protein in freshly isolated epidermal cells and cultured keratinocytes. Results from in vivo studies confirmed the presence of annexin I in the basal and suprabasal layers of normal human skin with modified reactivity patterns in hyperproliferative lesions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ando Y, Imamura S, Owada MK, Kakunaga T, Kannagi R (1989) Cross-linking of lipocortin 1 and enhancement of its Ca2+ sensitivity by tissue transglutaminase. Biochem Biophys Res. Commun 163: 944–951

    PubMed  Google Scholar 

  2. Becherucci C, Perretti M, Solito E, Galeotti CL, Parente L (1993) Conceivable difference in the anti-inflammatory mechanisms of lipocortins 1 and 5. Mediators Inflammation 2: 109–113

    Google Scholar 

  3. Bradford M (1986) A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72: 248–254

    Article  Google Scholar 

  4. Burgoyne RD, Geisow MJ (1989) The annexin family of Calcium-binding proteins. Cell Calcium 10: 1–10

    Article  PubMed  Google Scholar 

  5. Christmas P, Callaway J, Fallon J, Jones J, Haigler HT (1991) Selective secretion of annexin I, a protein without a signal sequence by the human prostate gland. J Biol Chem 266: 2499–2507

    PubMed  Google Scholar 

  6. Creutz CE, Zaks WJ, Hamman HC, Crane S, Martin WH, Gould KL, Oddie KM, Parsons SJ (1987) Identification of chromaffin granule binding proteins: relationship of the chromobinding to calelectrin, synhibin and the tyrosine kinase substrates p35 and p36. J Biol Chem 262: 1860–1868

    PubMed  Google Scholar 

  7. Creutz CE (1992) The annexins and exocytosis. Science 258: 924–930

    PubMed  Google Scholar 

  8. Crumpton MR, Moss SE, Crumpton MJ (1988) Diversity in the lipocortin/calpactin family. Cell 51: 1–3

    Article  Google Scholar 

  9. Culard JF, Basset-Seguin N, Calas B, Guilhou JJ, Martin F (1992) Characterization and subcellular localization of calcium-dependent phospholipid binding proteins (annexins) in normal human skin and reconstituted epidermis. J invest Dermatol 98: 436–441

    Article  PubMed  Google Scholar 

  10. Davidson FF, Dennis EA, Powell M, Glenney JR (1987) Inhibition of phospholipase A2 by lipocortins and calpactins. J Biol Chem 262: 1698–1705

    PubMed  Google Scholar 

  11. Dezutter-Dambuyant C, Schmitt D, Staquet MJ, Gaucherand M, Cambazard F, Thivolet J (1989) DMC1: a monoclonal antibody produced from histiocytosis X cells which reacts with the native CD1a molecule of human epidermal Langerhans cells. Hybridoma 8: 199–208

    PubMed  Google Scholar 

  12. Di Rosa M, Flower RJ, Hirata F, Parente L, Russo-Marie F (1984) Lipocortins, nomenclature announcement. Prostaglandins 28: 441–444

    Article  PubMed  Google Scholar 

  13. Fava RA, Cohen S (1984) Isolation of a calcium-dependent 35 kilodalton substrate for the epidermal growth factor receptor/kinase from A-431 cells. J Biol Chem 259: 2636–2645

    PubMed  Google Scholar 

  14. Fava RA, McKanna J, Cohen S (1989) Lipocortin 1 is abundant in a restricted number of differentiated cell types in adult organs. J Cell Physiol 141: 284–293

    PubMed  Google Scholar 

  15. Flower RS (1988) Lipocortin and the mechanism of action of glucocorticoids. Br J Pharmacol 94: 987–1015

    PubMed  Google Scholar 

  16. Geisow MJ, Walker JH (1986) New proteins involved in cell regulation by Ca2+ and phospholipids. Trends Biochem Sci 11: 420–423

    Article  Google Scholar 

  17. Goulding NJ, Guyre PM (1992) Regulation of inflammation by lipocortin. Immunol Today 13: 295–297

    Article  PubMed  Google Scholar 

  18. Goulet F, Moore KG, Sartorelli SC (1992) Glycosylation of annexin I and annexin II. Biochem Biophys Res Commun 188: 554–558

    PubMed  Google Scholar 

  19. Hegemann L, Mahrle G (1991) Biochemical pharmacology of protein kinase C and its relevance for dermatology. In: Mukhtar H (ed) Skin pharmacology. CRC Press, Boca Raton

    Google Scholar 

  20. Hullin F, Raynal P, Ragab-Thomas MF, Fauvel J, Chap H (1989) Effect of dexamethasone on prostaglandin synthesis and on lipocortin status in human endothelial cells. J Biol Chem 264: 3506–3513

    PubMed  Google Scholar 

  21. Khanna NC, Tokuda R, Waisman DM (1986) Phosphorylation of lipocortins in vitro by protein kinase C. Biochem Biophys Res Commun 141: 547–554

    PubMed  Google Scholar 

  22. Kitajima Y, Owada MK, Mitsui H, Yaoita H (1991) Lipocortin I (annexin I) is preferentially localized on the plasma membrane in keratinocytes of psoriatic lesional epidermis as shown by immunofluorescence microscopy. J Invest Dermatol 97: 1032–1038

    Article  PubMed  Google Scholar 

  23. Klee CB (1988) Ca2+-dependent phospholipid (and membrane) binding. Biochemistry 27: 6645–6653

    PubMed  Google Scholar 

  24. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of a bacteriophage T4. Nature 227: 680–685

    PubMed  Google Scholar 

  25. Peers SH, Flower RJ (1990) The role of lipocortin in cortico-steroid actions. Am Rev Respir Dis 141: 518–521

    Google Scholar 

  26. Perretti M, Flower RJ (1993) Modulation of IL-1-induced neutrophil migration by dexamethasone and lipocortin 1. J Immunol 150: 992–999

    PubMed  Google Scholar 

  27. Schlaepfer DD, Haigler HT (1987) Characterization of Ca2+-dependent phospholipid binding and phosphorylation of lipocortin I. J Biol Chem 262: 6931–6937

    PubMed  Google Scholar 

  28. Schlaepfer DD, Haigler HT (1990) expression of annexins as a function of cellular growth state. J Cell Biol 111: 229–238

    Article  PubMed  Google Scholar 

  29. Solito E, Parente L (1989) Modulation of phospholipase A2 activity in human fibroblasts. Br J Pharmacol 96: 656–660

    PubMed  Google Scholar 

  30. Solito E, Raugei G, Melli M, Parente L (1991) Dexamethasone induces the expression of mRNA of lipocortins 1 and 2 and the release of lipocortin 1 and 5 in differentiated but not in undifferenciated U937 cells. FEBS Lett 291: 238–244

    Article  PubMed  Google Scholar 

  31. Tamura GS, Dailey MO, Gallatin WM, McGrath S, Weissman IL, Pillemer EA (1984) Isolation of molecules recognized monoclonal antibodies and antisera. Anal Biochem 136: 458–464

    PubMed  Google Scholar 

  32. Viac J, Reano A, Brochier J, Staquet MJ, Thivolet J (1983) Reactivity pattern of a monoclonal anti-keratin antibody (KL1). J Invest Dermatol 81: 351–354

    Article  PubMed  Google Scholar 

  33. Viac J, Guerin-Reverchon M, Chignol MC, Chardonnet Y (1989) Filaggrin expression in cutaneous and mucosal human papillomavirus induced lesions. Pathol Res Pract 185: 342–346

    PubMed  Google Scholar 

  34. Violette SM, King I, Browning JL, Pepinsky RB, Wallner BP, Sartorelli AC (1990) Role of lipocortin I in the glucocorticoid induction of the terminal differentiation of a human squamous carcinoma. J Cell Physiol 142: 20–77

    Google Scholar 

  35. Wallner BP, Matterlino RJ, Hession C, Cate RA, Tizard R, Sinclair LK, Foeller C, Chow EP, Browning LJ, Ramachandran KL, Pepinsky RB (1986) Cloning and expression of human lipocortin, a phospholipase A2 inhibitor with potential antiinflammatory activity. Nature 320: 77–81

    PubMed  Google Scholar 

  36. Zokas L, Glenney JR (1987) The calpactin light chain is tightly linked to the cytoskeletal form of calpactin I: studies using monoclonal antibodies to calpactin subunits. J Cell Biol 105: 2111–2121

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. INSERM Unité 346, Clin. Dermatologique HÔpital E. Herriot, F-69437, Lyon Cedex, France

    M. Serres, J. Viac & D. Schmitt

  2. INRA, 180 Ch. de Tournefeuille, F-31300, Toulouse, France

    C. Comera

Authors
  1. M. Serres
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Viac
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Comera
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. D. Schmitt
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Serres, M., Viac, J., Comera, C. et al. Expression of Annexin I in freshly isolated human epidermal cells and in cultured keratinocytes. Arch Dermatol Res 286, 268–272 (1994). https://doi.org/10.1007/BF00387599

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00387599

Key words

Search

Navigation