Summary
Protein synthesis by chloroplasts isolated under aseptic conditions from Phaseolus vulgaris leaves is inhibited by the bacterial antibiotics spectinomycin, lincomycin, and erythromycin; that by chloroplasts from Nicotiana tabacum leaves is inhibited by spectinomycin and lincomycin but not by erythromycin. Protein synthesis by cytoplasmic ribosomes from plants and animals is not inhibited by these compounds, nor is amino acid activation by the soluble fraction from bean chloroplasts. These results suggest that chloroplast ribosomes possess sites which bind several unrelated bacterial antibiotics and support the idea that chloroplasts originated from prokaryotic cells. These antibiotics may be useful in studying the process of chloroplast formation in intact cells.
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Boardman, N. K., Francki, R. I. B., Wildman, S. G.: Protein synthesis by cell-free extracts from tobacco leaves II. Biochemistry 4, 872–876 (1965).
Bollen, A., Davies, J., Ozaki, M., Mizushima, S.: Ribosomal protein conferring sensitivity to the antibiotic spectinomycin in Escherichia coli. Science 165, 85–86 (1969).
Ellis, R. J.: Chloroplast ribosomes: stereospecificity of inhibition by chloramphenicol. Science 158, 477–478 (1969).
—, Macdonald, I. R.: Activation of protein synthesis by microsomes from aging beet disks. Plant Physiol. 42, 1297–1302 (1967).
Gualerzi, C., Cammarano, P.: Comparative electrophoretic studies on the protein of chloroplast and cytoplasmic ribosomes of spinach leaves. Biochim. biophys. Acta (Amst.) 190, 170–186 (1969).
Igarashi, K., Ishitsuka, H., Kaji, A.: Comparative studies on the mechanism of action of lincomycin, streptomycin and erythromycin. Biochem. biophys. Res. Commun. 37, 499–504 (1969).
Kirk, J. T. O., Tilney-Bassett, R. A. E.: The plastids. London: Freeman & Co. 1967.
Lamb, A. J., Clark-Walker, G. D., Linnane, A. W.: The biogenesis of mitochondria IV. Biochim. biophys. Acta (Amst.) 161, 415–427 (1968).
Linnane, A. W., Lamb, A. J., Christodoulou, G., Lukins, H. B.: The biogenesis of mitochondria VI. Proc. nat. Acad. Sci. (Wash.) 59, 1288–1293 (1968).
Loening, U. E., Ingle, J.: Diversity of RNA components in green plant tissues. Nature (Lond.) 215, 363–367 (1967).
Lyttleton, J. W.: Isolation of ribosomes from spinach chloroplasts. Exp. Cell Res. 26, 312–317 (1962).
Parenti, F., Margulies, M. M.: In vitro protein synthesis by plastids of Phaseolus vulgaris I. Plant Physiol. 42, 1179–1186 (1967).
Ranaletti, M., Gnanam, A., Jagendorf, A. T.: Amino acid incorporation by isolated chloroplasts. Biochim. biophys. Acta (Amst.) 186, 192–204 (1967).
Sagan, L.: On the origin of mitosing cells. J. theoret. Biol. 14, 225–274 (1967).
Schwartz, J. H., Meyer, R., Eisenstadt, J. M., Brawerman, G.: Involvement of N-formylmethionine in initiation of protein synthesis by cell-free extracts of Euglena gracilis. J. molec. Biol. 25, 571–574 (1967).
Spencer, D., Wildman, S. G.: The incorporation of amino acids into protein by cell-free extracts of tobacco leaves. Biochemistry 3, 954–959 (1964).
Stutz, E., Noll, H.: Characterisation of cytoplasmic and chloroplast polysomes in plants: evidence for three classes of ribosomal RNA in nature. Proc. nat. Acad. Sci. (Wash.) 57, 774–781 (1967).
Vazquez, D., Monro, R. E.: Effects of some inhibitors of protein synthesis on the binding of aminoacyl-tRNA to ribosomal subunits. Biochim. biophys. Acta (Amst.) 142, 155–173 (1967).
Weisblum, B., Davies, J.: Antibiotic inhibitors of the bacterial ribosome. Bact. Rev. 32, 493–528 (1968).
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Ellis, R.J. Further similarities between chloroplast and bacterial ribosomes. Planta 91, 329–335 (1970). https://doi.org/10.1007/BF00387506
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DOI: https://doi.org/10.1007/BF00387506