Abstract
Radiolabeled paraffinic and aromatic hydrocarbons, including benzopyrene, fluorene, naphthalene, methylnaphthalene, methylcholanthrene, hexadecane, heptadecane and dotriacontane, were taken up from food and water by the blue crab Callinectes sapidus. In 2 days, approximately 10% of the benzopyrene and fluorene were taken up from the water when their concentrations were 2.5 and 30 μg/l, respectively. When given food with radiolabeled hydrocarbons, 2 to 10% of the hydrocarbons were assimilated by the carbs, with the remainder excreted. After uptake of hydrocarbon from water or food, a major pathway for the elimination of hydrocarbon and metabolites was through fecal material. All hydrocarbons used in the study were metabolized, with similar rates for both paraffinic and aromatic hydrocarbons. More than 50% of the radioactivity assimilated by the crabs was in the hepatopancreas, suggesting that the hepatopancreas was the site of hydrocarbon metabolism. Twenty-five days aftex exposure to radiolabeled hydrocarbons, radioactivity was found only in the hepatopancreas. The hepatopancreas contained highly polar hydrocarbon metabolites, including dihydroxy-compounds and their conjugates, while blood contained both monohydroxy-and dihydroxy-compounds. No evidence was found of storage of hydrocarbons by any of the crab tissues.
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Literature Cited
Anderson, J. W., J. M. Neff, B. A. Cox, H. E. Tatem and G. M. Hightower: The effects of oil on estuarine animals; toxicity, uptake and depuration, respiration. In: Pollution and physiology of marine organisms, pp 285–310. Ed. by F. J. Vernberg and W. Vernberg, New York: Academic Press 1974
Bend, J. B., L. G. Hart, A. M. Guarino, D. P. Rall and J. R. Fouts: Distribution and excretion of 14C-2, 4, 5, 2′, 5′-pentachlorobiphenyl (14C-PCB) in the lobster (Homarus americanus) and the dogfish (Squalus acanthias). Pharmacologist 17, p. 196 (1974)
Blumer, M., G. Souza and J. Sass: Hydrocarbon pollution of edible shellfish by an oil spill. Mar. Biol. 5, 195–202 (1970)
Carlson, G. P.: Detoxification of foreign organic compounds by the quahaug, Mercenaria mercenaria. Comp. Biochem. Physiol. 43B, 295–302 (1972)
Clark, R. C. and J. S. Finley: Uptake and loss of petroleum hydrocarbons by mussels, Mytilus edulis, in laboratory experiments. Fish. Bull. U. S. 73, 508–515 (1975)
Conney, A. H. and J. J. Burns: Metabolic interaction among environmental chemicals and drugs. Science, N. Y. 178, 576–586 (1972)
Corner, E. D. S., R. P. Harris, C. C. Kilvington and S. C. M. O'Hara: Petroleum compounds in the marine food web: short-term experiments on the fate of naphthalene in Calanus. J. mar. biol. Ass. U. K. 56, 121–133 (1976)
—, C. C. Kilvington and S. C. M. O'Hara: Qualitative studies on the metabolism of naphthalene in Maia squinada (Herbst). J. mar. biol. Ass. U. K. 53, 819–832 (1973)
Costlow, J. D. and D. G. Bookhout: The larval development of Callinectes sapidus Rathbun reared in the laboratory. Biol. Bull. mar. biol. Lab., Woods Hole 116, 373–396 (1959)
Cox, B. A., J. W. Anderson and J. C. Parker: An experimental oil spill: the distribution of aromatic hydrocarbons in the water, sediment, and animal tissues within a shrimp pond. In: Proceedings of the 1975 Conference of Prevention and Control of oil Pollution, pp 607–612. Washington, D. C.: American Petroleum, Institute 1975
Darnell, R. M.: Studies of the life history of the blue crab (Callinectes sapidus Rathbun) in Louisiana waters. Trans. Am. Fish. Soc. 88, 294–304 (1959)
Ehrhardt, M.: Petroleum hydrocarbons in oysters from Galveston Bay. Envir. Pollut. 3, 257–271 (1972)
Elmamlouk, T. H., T. Gessner, and A. C. Brownie: Occurrence of cytochrome P-450 in hepatopancreas of Homarus americanus. Comp. Biochem. Physiol. 48, 419–425 (1972)
Farrington, J. W. and J. G. Quinn: Petroleum hydrocarbons in Narragansett Bay. I. Survey of hydrocarbons in sediments and clams (Mercenaria mercenaria). Estuar. cstl mar. Sci. 1, 71–79 (1973)
Folch, J., M. Lees and G. H. Sloane Stanley: A simple method for the isolation and purification of total lipids from animal tissues. J. biol. Chem. 226, 497–509 (1957)
Fossato, V. U. and E. Siviero: Oil pollution monitoring in the Lagoon of Venice using the mussel Mytilus galloprovincialis. Mar. Biol. 25, 1–6 (1974)
Gelboin, H. V., N. Kinoshita and F. J. Wiebel: Microsomal hydroxylases: induction and role in polycyclic hydrocarbon carcinogenesis and toxicity. Fedn Proc. Fedn Am. Socs exp. Biol. 31, 1298–1309 (1972)
Guarino, A. M., J. B. Pritchard, J. B. Call, H. A. Bering and D. P. Rall: Distribution of 14C-DDT in the lobster after administration via intravascular or oral routes or after exposure to DDT-containing sea water. Bull. Mt Desert Isl. biol. Lab. 13, 29–31 (1972)
Hodgson, E.: Comparative studies of cytochrome P-450 and its interactions with pesticides. In: Survival in toxic environments, pp 213–255. Ed by M. A. Q. Khan and J. P. Bederka. New York: Academic Press 1974
Khan, M. A. Q., W. Coello, A. A. Khan and H. Pinto: Some characteristics of the microsomal mixedfunction oxidase in the freshwater crayfish, Cambarus. Life Sciences 11, 405–415 (1972)
Lee, R. F.: Fate of petroleum hydrocarbons in marine zooplankton. In: Proceedings of the 1975 Conference on Prevention and Control of Oil Pollution, pp 549–553. Washington, D. C.: American Petroleum Institute 1975
—, R. Sauerheber and A. A. Benson: Petroleum hydrocarbons: Uptake and discharge by the marine mussel Mytilus edulis. Science, N. Y., 177, 344–346 (1972a)
—— and G. H. Dobbs: Uptake, metabolism and discharge of polycyclic hydrocarbons by marine fish. Mar. Biol. 17, 201–208 (1972b)
Neff, J. M. and J. W. Anderson: Accumulation, release and distribution of benzo (a) pyrene-C14 in the clam Rangia cuneata. In: Proceedings of the 1975 Conference on Prevention and Control of Oil Pollution, pp 469–472. Washington, D. C.: American Petroleum Institute 1975
Nicol, J. A. C.: The biology of marine animals, 699 pp. New York: John Wiley & Sons 1967
Nimmo, D. R., A. J. Wilson and R. R. Blackman: Localization of DDT in the body organs of pink and white shrimp. Bull. envir. Contam. Toxicol. 5, 333–341 (1970)
Pohl, R. J., J. R. Bend, A. M. Guarino and J. R. Fouts: Hepatic microsomal mixed-function oxidase of several marine species from coastal Maine. Drug Metab. Disposition 2, 545–555 (1974)
Stegeman, J. J.: Hydrocarbons in shellfish chronically exposed to low levels of fuel oil. In: Pollution and physiology of marine organisms, pp 329–347. Ed. by F. J. Vernberg and W. B. Vernberg. New York: Academic Press 1974
— and J. M. Teal: Accumulation, release and retention of petroleum hydrocarbons by the oyster Crassostrea virginica. Mar. Biol. 22, 37–44 (1973)
Tagatz, M. E.: Biology of the blue crab, Callinectes sapidus Rathbun, in the St. Johns River, Florida. Fishery Bull. Fish Wildl. Serv. U. S. 67, 17–33 (1968)
Van Engel, W. A.: The blue crab and its fishery in Chesapeake Bay. Part 1-Reproduction, early development, growth and migration. Comml Fish. Rev. 20, 6–17 (1958)
White, D. B., R. R. Stickney, D. Miller and L. H. Knight: Sea water system for aquaculture of estuarine organisms at the Skidaway Institute of Oceanography. Tech. Rep. Ser. Ga mar. Sci. Cent. Savannah, Ga 73-1, 1–18 (1973)
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Communicated by M. R. Tripp, Newark
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Lee, R.F., Ryan, C. & Neuhauser, M.L. Fate of petroleum hydrocarbons taken up from food and water by the blue crab Callinectes sapidus . Marine Biology 37, 363–370 (1976). https://doi.org/10.1007/BF00387492
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DOI: https://doi.org/10.1007/BF00387492