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Quantitative ion localization within Suaeda maritima leaf mesophyll cells

Planta volume 151, pages 555–560 (1981)Cite this article

Abstract

Grown under saline conditions, Suaeda maritima accumulates Na+ and Cl- into its leaves, where individual mesophyll cells behave differently in their compartmentation of these ions. Measurements of ion concentrations within selected subcellular compartments show that freeze-substitution with dry sectioning is a valuable preparative technique for analytical electron microscopy of highly vacuolate plant material. Using this approach, absolute estimates were made of Na+, K+ and Cl- concentrations in the cytoplasm, cell walls, chloroplasts and vacuoles of leaf mesophyll cells.

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Abbreviations

TAEM:

transmission analytical electron microscopy

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Author information

Affiliations

  1. School of Biological Sciences, University of Sussex, BN1 9QG, Brighton, Sussex, UK

    Diana M. R. Harvey, J. L. Hall & T. J. Flowers

  2. National Physical Laboratory, TW11 OL W, Teddington, Middlesex, UK

    B. Kent

Authors
  1. Diana M. R. Harvey
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  2. J. L. Hall
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  3. T. J. Flowers
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  4. B. Kent
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Harvey, D.M.R., Hall, J.L., Flowers, T.J. et al. Quantitative ion localization within Suaeda maritima leaf mesophyll cells. Planta 151, 555–560 (1981). https://doi.org/10.1007/BF00387435

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  • DOI: https://doi.org/10.1007/BF00387435

Key words

  • Halophyte
  • Ion accumulation
  • Suaeda