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Mechanical stability of some immobilised preparations for glucose isomerisation in fixed and agitated bed columns

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Abstract

Immobilised preparations mechanical stability was studied by an analysis of particle disintegration in fixed and agitated beds of laboratory reactors. The analysis was carried out by comparing the particle size distributions obtained for three different states within the overall time-course of operation: The initial state, an intermediate state and the final one. It was found that mechanical stability by both fixed bed operation and paddle mixing was high. Agitation by flat discs was shown to cause intensive particle attrition. The relationships observed are discussed with regard to the flow pattern of the various types of reactors.

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References

  1. Reilly, P. J.: Enzymic isomerisation of corn starch-based glucose syrups. In: Van Beynum, G. M. A.; Roels, J. A. (Eds.): Starch conversion technology, pp. 175–198. Marcel Dekker 1985

  2. Venkatasubramanian, K.; Karkare, S. B.; Vieth, W. R.: Chemical engineering analysis of immobilised-cell systems. In: Chibata, I.; Wingard, L. B. (Eds.): Immobilised microbial cells, Vol. 4, pp. 311–349. Academic Press 1983

  3. NOVO Sweetzyme production package, IB-No. 175c-GB (1982)

  4. Webb, C.; Dervakos, G. A.; Dean, J. F.: The importance of mixing in immobilised cell systems. Proc. 2nd Int. Conf. on Bioreactor Fluid Dynamics, Cambridge, Sept. 1988, pp. 199–213, BHRA, Cranfield 1985

    Google Scholar 

  5. Luyben, K. Ch. A. M.: Continuous multistage fluid-bed reactor for immobilized biocatalyst. Presented at: Symposium Interbiotech'89 Bratislava, 28–30 June, 1989

  6. Product information R 846, Miles Biotech Products Division, 1987

  7. Djejeva, G.: Glucose isomerase in Streptomyces strains — some theoretical and applied aspects. DSc Thesis, Bulg. Acad. Sci. 1990

  8. Norsker, O.; Gibson, K.; Zittan, L.: Experience with empirical methods for evaluating pressure drop properties. Starch 31 (1979) 13–16

    Google Scholar 

  9. Nagata, S.; Yamamoto, K.; Ujihara, M.: Flow patterns of liquid in a cylindrical mixing vessel without baffles. Mem. Fac. Eng. Kyoto Univ. 20 (1958) 336–349

    Google Scholar 

  10. Brauer, H.: Power consumption in aerated stirred tank reactor systems, Adv. Biochem. Eng. 13 (1979) 87–119

    Google Scholar 

  11. Vlaev, S. D.; Valeva, M.: An extension of the pressure-test technique for immobilised biocatalyst preparations (in press)

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Vlaev, S.D., Elenkov, D. & Tsenova, M. Mechanical stability of some immobilised preparations for glucose isomerisation in fixed and agitated bed columns. Bioprocess Engineering 7, 189–192 (1991). https://doi.org/10.1007/BF00387416

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  • DOI: https://doi.org/10.1007/BF00387416

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