Abstract
In order to improve the cultivation properties of a traditional continuous stirred tank reactor (CSTR), we introduced a circulation unit made of four inorganic membranes in stainless steel tubes in parallel configuration, the so-called Tubular Bioreactor (TBR). Furthermore, the TBR outlet tube, which has a restriction nozzle at the end, was installed on top of the fermentor vessel, thereby creating a strong jet flow into the reactor and thus improving the mixing and the oxygen transfer rate. The k La could be increased by approximately 50%. This setup was used for cultivations of recombinant Escherichia coli in a minimal medium and high cell density. More than 50 g dry cell mass/dm3 was obtained. Simultaneously, we have produced an elongated form of human insulin-like growth factor II, which was a secreted fusion protein utilizing the E. coli secretion system based on staphylococcus protein A. The product could be recovered continuously through the TBR-membrane.
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References
Bin, A. K.: Gas entrainment by plunging liquid jets. VDI Forschungsheft 648 (1988) 1–36
Landwall, P.; Holme, T.: Removal of inhibitors of bacterial growth by dialysis culture. J. Gen. Microb. 103 (1977) 345–352
Landwall, P.; Holme, T.: Influence of glucose and dissolved oxygen concentrations on yields of Escherichia coli B in dialysis cultur. J. Gen. Microb. 103 (1977) 353–358
Paalme, T.; Tiisma, A.; Kahru, K.; Vanatalu, K.; Vilu, R.: Glucose-limited fed-batch cultivation of Escherichia coli with computer controlled fixed growth rate. Biotech. Bioeng. 35 (1990) 312–319
Endo, I.; Nagamune, T.; Tachikawa, S.; Inaba, H.: Tubular bioreactor for high density cultivation of microorganisms. In: Fiechter, A. (Ed.): Advance in Biochem. Eng./Biotech. Heidelberg, New York, Berlin: Springer-Verlag, in press.
Abrahmsén, L.; Moks, T.; Nilsson, B.; Uhlén, M.: Secretion of heterologous gene products to the culture medium of Escherichia coli. Nucl. Acids Res. 14 (1986) 7487–7500
Nilsson, B.; Moks, T.; Jansson, B.; Abrahmsén, L.; Elmblad, A.; Holmgren, E.; Henrichson, C.; Jones, T. A.; Uhlén, M.: A synthetic IgG-binding domain based on staphylococcal protein A. Prot. Eng. 1 (1987) 107–113
Hammarberg, B.; Moks, T.; Tally, M.; Elmblad, A.; Holmgren, E.; Murby, M.; Nilsson, B.; Josephson, S.; Uhlén, M.: Differential stability of recombinant human insulin-like growth factor II in Escherichia coli and Staphylococcus aureaus. J. Biotech. 14 (1990) 423–438
Hammarberg, B.; Tally, M; Samuelsson, E.; Wadensten, H.; Holmgren, E.; Hartmanis, M.; Uhlén, M.; Moks, T.: Characterization of an extended form of recombinant human insulin-line growth factor II. in press J. Biol. Chem.
Gergen, P. J.; Stern, R. H.; Wensink, P. C.: Filter replicas and permanent collections of recombinant DNA plasmids. Nucl. Acid Res. 7 (1979) 2115–2136
Moks, T.; Abrahmsén, L.; Österlöf, B.; Josephson, S.; Östling, M.; Enfors, S.-O.; Persson, I.; Nilsson, B.; Uhlén, M.: Large-scale affinity purification of human insulin-like growth factor I from culture medium of Escherichia coli. Bio/Tech 5 (1987) 379–382
Nygren, P.-Å.; Eliasson, M.; Abrahmsén, L.; Uhlén, M.: Palmcrantz, E.: Analysis and use of the serum albumin binding domains of Streptococcal protein G. J. Mol. Recog. 1 (1988) 69–74
Taddei, C.; Daufin, G.; Aimar, P.; Sanchez, V.; Role of some whey components on mass transfer in ultrafiltration. Biotech. Bioeng. 34 (1989) 171–179
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Hammarberg, B., Nagamune, T., Endo, I. et al. Utilizing the tubular bioreactor for continuous recovery of secreted fusion protein from recombinant Escherichia coli. Bioprocess Engineering 7, 145–150 (1991). https://doi.org/10.1007/BF00387409
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DOI: https://doi.org/10.1007/BF00387409