Summary
SO --3 inhibits the activity of ribulose-1,5-diphosphate carboxylase in isolated spinach chloroplasts. It shows a non-competitive inhibition pattern with respect to ribulose-1,5-diphosphate and Mg++ but a competitive one with respect to HCO -3 . The K i -values are 14 mM SO --3 and 9.5 mM SO -3 respectively for the non-competitive inhibition but only 3.0 mM SO --3 in the case of competitive inhibition with HCO --3 as a substrate. Thus it is concluded that the competitive inhibition type will predominate at low SO --3 and low internal CO2 concentrations.
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Abbreviations
- RuDph:
-
ribulose-1,5-diphosphate
- DTT:
-
dithiothreitol
- EDTA:
-
ethylenediaminetetraacetate
References
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Ziegler, I. The effect of SO --3 on the activity of ribulose-1,5-diphosphate carboxylase in isolated spinach chloroplasts. Planta 103, 155–163 (1972). https://doi.org/10.1007/BF00387367
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DOI: https://doi.org/10.1007/BF00387367