Summary
-
1.
Of the buffer substances introduced by Good, only N-2-hydroxy-ethylpiperazine-N-2-ethanesulfonic acid (HEPES) and N-tris(hydroxymethyl)-methyl-2-aminoethanesulfonic acid (TES) were found suitable for the isolation of mitochondria by means of density gradient centrifugation in a silica sol. Addition of 1% polyvinylpyrrolidone remarkably reduced the respiration rate and diminished the respiratory control, whereas 2% dextran improved the rates.
-
2.
Fifty mM malonate inhibited succinate oxidation by 55–72% compared with the controls; in addition an uncoupling effect was observed. Similar results were obtained with 2.5 mM amytal.
-
3.
Antimycin A, in contrast, did not affect the respiratory control, while the respiration rate was depressed to 55% of that in the control.
-
4.
Azide (20 mM) had only a slight effect on the two parameters of leaf mitochondria.
-
5.
It can be concluded that mitochondria of leaf cells are provided with the same respiratory enzymes as those of other plant materials.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- TES:
-
N-Tris(hydroxymethyl)-methyl-2-aminoäthansulfonsäure
- MES:
-
2-Morpholinäthansulfonsäure
- TAPS:
-
N-Tris(hydroxymethyl)-methyl-3-aminopropansulfonsäure
- HEPES:
-
N-2-Hydroxyäthylpiperazin-N-2-äthansulfonsäure
Literatur
Arnon, D. I.: Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol. 24, 1–15 (1949).
Chance, B., Hackett, D. P.: The electron transfer system of skunk cabbage mitochondria. Plant Physiol. 34, 33–48 (1959).
—, Williams, G. R.: The respiratory chain and oxidative phosphorylation. Advanc. Enzymol. 17, 65–134 (1956).
Good, N. E., Winget, G. D., Winter, W., Conolly, T. N., Izawa, S., Singh, R. M. M.: Hydrogen ion buffers for biological research. Biochemistry 5, 467–477 (1966).
Gronebaum-Turck, K., Willenbrink, J.: Isolierung und Eigenschaften von Mitochondrien aus Blättern von Spinacia oleracea und Beta vulgaris. Planta (Berl.) 100, 337–346 (1971).
Hackett, D. P.: Respiratory mechanisms in higher plants. Ann. Rev. Plant Physiol. 10, 113–146 (1959).
—, Haas, D. W., Griffiths, S. K., Niederpruem, D. J.: Studies on development of cyanid-resistant respiration in potato tuber slices. Plant Physiol. 35, 8–19 (1960).
Ikuma, H., Bonner, W. D., Jr.: Properties of higher plant mitochondria. 3. Effects of respiratory inhibitors. Plant Physiol. 42, 1535–1544 (1967).
Jones, J. D., Hulme, A. C., Wooltorton, L. S. C.: The use of PVP in the isolation of enzymes from apple fruits. Phytochemistry 4, 659–676 (1965).
Lieberman, M.: Oxydative activity of cytoplasmatic particles of apples: electron transfer chain. Plant Physiol. 35, 796–801 (1960).
Packer, L., Murakami, S., Mehard, C. W.: Ion transport in chloroplasts and plant mitochondria. Ann. Rev. Plant Physiol. 21, 271–304 (1970).
Pertoft, H., Bäck, O., Lindahl-Kiessling, K.: Separation of various blood cells in colloidal silica-polyvinylpyrrolidone gradients. Exp. Cell Res. 50, 355–368 (1968).
Srivastava, H. K., Sarkissian, I. V.: Properties of wheat mitochondria. Study of substractes, cofactors and inhibitors. Physiol. Plantarum (Cph.) 23, 63–74 (1970).
Stinson, R. A., Spencer, M.: An evaluation of the effects of five buffers on respiratory parameters of isolated mitochondria. Canad. J. Biochem. 46, 43–50 (1968).
Wiskich, J. T., Bonner, W. D., Jr.: Preparation and properties of sweet potato mitochondria. Plant Physiol. 38, 594–604 (1963).
Author information
Authors and Affiliations
Additional information
Teil einer Diplomarbeit.
Rights and permissions
About this article
Cite this article
Nordhorn, G., Willenbrink, J. Hemmung einiger Atmungsenzyme in Mitochondrien aus grünen Blättern. Planta 103, 147–154 (1972). https://doi.org/10.1007/BF00387366
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00387366