Abstract
β-Glucosidase activity for coniferin (coniferyl alcohol β-D-glucoside) is not present in spruce (Picea abies L. Karst.) seeds but appears in the young seedlings. Lignification starts at ca. day 9 of germination in the vascular bundles. An antiserum against glucosidase 1 isolated from spruce seedlings (Marcinowski and Grisebach, Eur J. Biochem. 1978) was employed for the localization of the enzyme in cross sections of hypocotyls using immunofluorescent technique. The results indicate that at this stage of development the glucosidase is localized at the inner layer of the secondary cell wall. Glucosidase activity was present in all cells of the investigated hypocotyl tissue.
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Ashford, A.E.: Histochemical localization of β-glycosidases in roots of Zea mays. I. A simultaneous coupling azo-dye technique for the localization of β-glucosidase and β-galactosidase, Protoplasma 71, 281–293 (1970)
Ashford, A.W., McCully, M.E.: Histochemical localization of β-glycosidases in roots of Zea mays. II. Changes in localization and activity of β-glucosidase in the main root apex. Protoplasma 71, 389–402 (1970)
Cohen, R.B., Rutenburg, S.H., Tson, K.C., Woodburg, W.S., Seligmann, A.M.: The colorimetric estimation of β-D-glucosidase, J. Biol. Chem. 195, 607–614 (1952)
Feteanu, A.: Labelled antibodies in biology and medicine. New York: Mc. Graw Hill 1978
Freudenberg, A., Reznik, H., Boesenberg, H., Rasenack, D.: Das an der Verholzung beteiligte Fermentsystem. Chem. Ber. 85, 641–647 (1952)
Freudenberg, W., Reznik, H., Fuchs, W., Reichert, M.: Untersuchungen über die Entstehung des Lignins und des Holzes. Naturwissenschaften 42, 29–35 (1955)
Gierse, H.D., Barz, W.: Ein fluoreszenzmikroskopischer Nachweis von β-Glucosidase in Wurzeln von Cicer arietinum (L.), Protoplasma 88, 1–6 (1976)
Goldman, M.: Fluorescent antibody methods, New York: Academic Press, 1968
Hattersley, P.W., Watson, L., Osmond, C.B.: In situ immunofluorescent labelling of ribulose-1,5-bisphosphate carboxylase in leaves of C3 and C4 plants. Aust. J. Plant Physiol. 4, 523–539 (1977)
Hirschfeld, J.: Immunoelectrophoresis — procedure and application to the study of group — specific variations in sera, Sci. Tools 7, 18–25 (1960)
Hösel, W., Surholt, E., Borgmann, E.: Characterization of β-glucosidase isoenzymes possibly involved in lignification from chick pea (Cicer arietium L.) cell suspension cultures, Eur. J. Biochem. 84, 487–492 (1978)
Marcinowski, S., Grisebach, H.: Turnover of Coniferin in Spruce Seedlings. Phytochemistry 16, 1665–1667 (1977)
Marcinowski, S., Grisebach, H.: Enzymology of lignification. Cell wall-bound β-glucosidases for coniferin from spruce (Picea abies) seedlings, Eur. J. Biochem. 87, 37–44 (1978)
Munoz, J.: Double Diffusion in plates. In: Methods in Immunology and Immunochemistry Vol. III, pp. 146–160. Academic Press New York: 1971
Pickett-Heaps, J.D.: Xylem wall deposition. Radioautographic investigations using lignin precursors, Protoplasma 65, 181–205 (1968)
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Marcinowski, S., Falk, H., Hammer, D.K. et al. Appearence and localization of a β-glucosidase hydrolyzing coniferin in spruce (Picea abies) seedlings. Planta 144, 161–165 (1979). https://doi.org/10.1007/BF00387265
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DOI: https://doi.org/10.1007/BF00387265