Summary
The kinetics of chlorophyll formation during the greening of dark-grown Euglena gracilis was accompanied by marked increases in activity of the enzymes of glycollate metabolism; glycollate: DCPIP1 oxidoreductase and phosphoglycollate phosphatase (E.C. 3.1.3.18.). Inhibitors of protein synthesis indicated a de novo synthesis of these enzymes during the development of the photosynthetic system. The inhibitory effects of chloramphenicol and cycloheximide, together with a non-aqueous localisation of glycollate: DCPIP oxidoreductase in photoautotrophically-grown cells, indicated that this enzyme is synthesized and located in the cytoplasm, while phosphoglycollate phosphatase was synthesized in the chloroplast. Glycollate: DCPIP oxidoreductase did not increase above the low level in heterotrophic cells when exogenous glycollate, in the presence or absence of glucose, was supplied in the dark.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anderson, D. E., Tolbert N. E.: Phosphoglycollate phosphatase. Methods in Enzymology, vol. 9, p. 646–650, ed. W. A. Wood. New York: Academic Press 1966.
Arnon, D. I.: Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiol. 24, 1–13 (1949).
Benson, A. A., Calvin, M.: The path, of carbon in photosynthesis. VII. Respiration and photosynthesis. J. exp. Bot. 1, 63–68 (1950).
Codd, G. A., Lord, J. M., Merrett, M. J.: The glycollate oxidising enzyme of algae. FEBS Lett. 5, 341–342 (1969).
Cramer, M., Myers, J.: Growth and photosynthetic characteristics of Euglena gracilis. Arch. Mikrobiol. 17, 384–402 (1952).
Hess, J. L., Tolbert, N. E.: Glycolate pathway in algae. Plant Physiol. 42, 371–379 (1967).
Hutner, S. J., Bach, M. K., Ross, G. I.: A sugar-containing basal medium for vitamin B12 assay with Euglena; application to body fluids. J. Protozool. 3, 101–112 (1956).
Lord, J. M., Merrett, M. J.: Glycollate oxidase in Chlorella pyrenoidosa. Biochim. biophys. Acta (Amst.) 159, 543–544 (1968).
——: The pathway of glycollate utilization in Chlorella pyrenoidosa. Biochem. J. 117, 929–937 (1970).
Lowry, O. H., Rosebrough, N. J., Farr, A.L., Randall, R. J.: Protein measurements with the folin phenol reagent. J. biol. Chem. 193, 265–275 (1951).
Lynch, V. H., Calvin, M.: CO2 fixation by Euglena. Ann. N. Y. Acad. Sci. 56, 890–900 (1953).
Smillie, R. M.: Formation and function of soluble proteins in chloroplasts Canad. J. Bot. 41, 123–154 (1963).
— Evans, W. R., Lyman, H.: Metabolic events during the formation of a photosynthetic from a non-photosynthetic cell. Brookhaven Symp. Biol. 16, 89–108 (1963).
— Graham, D., Dwyer, M. R., Grieve, A., Tobin, N. F.: Evidence for the synthesis in vivo of proteins of the Calvin cycle and of the photosynthetic electrontransfer pathway on chloroplast ribosomes. Biochem. biophys. Res. Commun. 28, 604–610 (1967).
Thompson, C. M., Whittingham, C. P.: Intracellular localisation of phosphoglycollate phosphatase and glyoxylate reductase. Biochim. biophys. Acta (Amst.) 143, 642–644 (1967).
Tolbert, N. E., Cohan, M. S.: Activation of glycolic acid oxidase in plants. J. biol. Chem. 204, 639–648 (1953).
— Zill, L. P.: Excretion of glycollic acid by algae during photosynthesis. J. biol. Chem. 222, 895–906 (1956).
Whittingham, C. P., Pritchard, G. G.: The production of glycollate during photosynthesis in Chlorella. Proc. roy. Soc. B 157, 366–382 (1963).
Zelitch, I., Day, P. R.: Glycolate oxidase activity in algae. Plant Physiol. 43, 289–291 (1968).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Codd, G.A., Merrett, M.J. Enzymes of the glycollate pathway in relation to greening in Euglena gracilis . Planta 95, 127–132 (1970). https://doi.org/10.1007/BF00387245
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00387245