Summary
From 250 kg of fresh chicory roots about 2 mg of a crystalline cytokinin were obtained. This substance was identified as ribosylzeatin (trans isomer). From the procedure employed it seems unlikely that the isolated cytokinin comes from the degradation of tRNAs; rather, it may constitute a separate pool of cytokinins.
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References
Buchanan, J. G., Dekker, A. A., Long, A. G.: The detection of glycosides and non-reducing carbohydrate derivatives in paper partition chromatography. J. chem. Soc. 1950, 3162–3167.
Bui Dang Ha., D.:Recherches sur les methodes de purification des cytokinines naturelles. Isolement d'une cytokinine à partir de la racine d'endive (Cichorium intybus L.). Doct. Dissert., Fac. Sci. Paris, 68 pp., 1970.
Carr, D. J., Burrows, W. J.: Evidence of the presence in xylem sap of substances with kinetin-like activity. Life Sci. 5, 2061–2077 (1966).
—, Reid, D. M.: The physiological significance of the synthesis of hormones in roots and of their export to the shoot system. In: Biochemistry and physiology of plant growth substances (F. Wightman and G. Setterfield, eds.), p. 1169–1185, Ottawa: Runge Press 1968.
Gautheret, R. J.: Recherches expérimentales sur la polarité des tissus de la racine d'Endive. C. R. Acad. Sci. (Paris) 213, 37–39 (1941).
Hall, R. H., Csonka, L., Davis, H., McLennan, B.: Cytokinins in the soluble RNA of plant tissues. Science 156, 69–71 (1967).
—, Ropp, R. S. de: Formation of 6-furfurylaminopurine from DNA breakdown products. J. Amer. chem. Soc. 77, 6400 (1955).
Hecht, S. M., Leonard, N. J., Burrows, W. J., Armstrong, D. J., Skoog, F., Occolowitz, J.: Cytokinin of wheat germ transfer RNA: 6-(4-hydroxy-3-methyl-2-butenylamino)-2-methylthio-9-β-D-ribofuranosylpurine. Science 166, 1272–1274 (1969).
Helgeson, J. P.: The cytokinins, Science 161, 974–981 (1968).
Kende, H.: Preservation of chlorophyll in leaf sections by substances obtained from root exudate. Science 145, 1066–1067 (1964).
—: Kinetin-like factors in the root exudate of sunflowers. Proc. nat. Acad. Sci. (Wash.) 53, 1302–1304 (1965).
Kulaeva, O. N.: The effect of roots on leaf metabolism in relation to the action of kinetin on leaves. Fiziol. Rast. 9, 229–239 (1962).
Letham, D. S.: Purification and probable identity of a new cytokinin in sweet corn extracts. Life Sci. 5, 551–554 (1966).
—: A new cytokinin bioassay and the naturally-occurring cytokinin complex. In: Biochemistry and physiology of plant growth substances (F. Wightman and G. Setterfield, eds.) p. 19–31. Ottawa: Runge Press 1968.
Loeffler, J. E., Overbeek, J. van: Kinin activity in coconut milk. In: Régulateurs Naturels de la Croissance Végétale, J. P. Nitsch ed., p. 77–82. Paris: C.N.R.S. 1963.
Most, B. H., Williams, J. C., Parker, K. J.: Gas chromatography of cytokinins. J. Chromat. 38, 136–138 (1968).
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plantarum (Cph.) 15, 473–497 (1962).
Nitsch, J. P., Bui Dang Ha, D., Nitsch, C.: Extraction d'un, facteur de bourgeonnement de l'endive (Cichorium intybus L.). Bull. Soc. bot. Fr. 113, 425–429 (1966).
— Nitsch, C.: Action synergique des auxines et du jus de tomate sur la croissance de tissus végétaux cultivés in vitro. Bull. Soc. bot. Fr. 102, 519–527 (1955).
——: Presence de cytokinines et autres substances de croissance dans la sève d'Acer saccharum et de Vitis vinifera. Bull. Soc. bot. Fr. 112, 11–18 (1965).
Parthier, B.: Proteinsynthese in grünen Blättern. II. Aminosäure-Inkorporation in die Protein subzellulärer Strukturen in Abhängigkeit vom physiologischen Zustand der Blätter. Flora 154, 230–244 (1964).
Reid, D. M., Burrows, W. J.: Cytokinin and gibberellin-like activity in the spring sap of trees. Experientia (Basel) 24, 189–190 (1968).
Rogozinska, J. H., Helgeson, J. P., Skoog, F.: Tests for kinetin-like growth promoting activities of triacanthine and its isomer, 6-(γ, γ-dimethylallylamino)-purine. Physiol. Plantarum (Cph.) 17, 164–176 (1964).
Schouteden-Wery, J.: Quelques expériences de régénération des bourgeons chez les racines de Chicorée. Bull. Acad. Roy. Belgique, Cl. Sci. V 6, 152–166 (1920).
Seth, A., Wareing, P. F.: Isolation of a kinin-like root-factor in Phaseolus vulgaris. Life Sci. 4, 2275–2280 (1965).
Shaw, G., Smallwood, B. M., Wilson, D. V.: Purine, pyrimidines, and imidazoles. XXIV. Synthesis of zeatin, a naturally occuring adenine derivative with plant cell-division-promoting activity, and its 9-β-D-ribofuranoside. J. chem. Soc. 1966(c) 921–924.
Skoog, F., Armstrong, D. J.: Cytokinins. Ann. Rev. Plant Physiol. 21, 359–384 (1970).
—: Chemical regulation of growth and organ formation in plant tissues-cultured in vitro. Symp. Soc. exp. Biol. 11, 118–130 (1957).
Vardjan, M., Nitsch, J. P.: La régénération chez Cichorium endivia L.: étude des auxines et des “kinines” endogènes. Bull. Soc. bot. Fr. 108, 363–374 (1961).
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Bui-Dang-Ha, D., Nitsch, J.P. Isolation of zeatin riboside from the chicory root. Planta 95, 119–126 (1970). https://doi.org/10.1007/BF00387244
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DOI: https://doi.org/10.1007/BF00387244