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The transport and metabolism of 14C-labelled indoleacetic acid in intact pea seedlings

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Summary

Part of the IAA-I- or IAA-2-14C applied at low concentrations to the apices of intact, light-grown dwarf pea seedling was transported unchanged to the root system The calculated velocity of transport in the stem was 11 mm per hour. In the root the label accumulated in the developing lateral root primordia.

A large proportion of the applied IAA was converted by tissues of the apical bud, stem and root to indole-3-acetyl-aspartic acid (IAAsp). This compound was not transported. In addition evidence was obtained for the formation of IAA-protein complexes in the apex and roots, but not in the fully-expanded internodes.

Large quantities of a decarboxylation product of IAA, tentatively indentified as indole-3-aldehyde (IAld), and several minor metabolites of IAA, were detected in extracts of the roots and first internodes, but not in the above-ground organs exposed to light. These compounds were readily transported through stem and root tissues. Together, the decarboxylation of IAA and the formation of IAAsp operated to maintain a relatively constant level of free IAA-14C in the root system.

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Author notes

  1. R. E. Briant

    Present address: Department of Biological Sciences, Wye College, Ashford, Kent

  2. P. G. Thomson

    Present address: Agricultural Divison, Shell International Chemical Co. Ltd., Shell Centre, S. W. I., London

Authors and Affiliations

  1. Department of Botany, University of Southampton, UK

    D. A. Morris, R. E. Briant & P. G. Thomson

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  3. P. G. Thomson
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Morris, D.A., Briant, R.E. & Thomson, P.G. The transport and metabolism of 14C-labelled indoleacetic acid in intact pea seedlings. Planta 89, 178–197 (1969). https://doi.org/10.1007/BF00386984

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