Summary
The enhancement by indoleacetic acid (IAA) of 36Cl- uptake into Avena coleoptile sections was used to study the effects of a hormone on a membrane-controlled phenomenon. Compared to sections in phosphate buffer only, Cl- content of the cells increases 15 min after addition of IAA; the promotion is seen only with growth-active auxins and is saturated at 3 μM IAA. The percent enhancement by IAA is the same over a wide range of Cl- concentrations. The hormone effect is not observed at ice-bath temperature and is not correlated with growth or water movement into the cells. IAA does not influence the movement of Cl- in the section. While auxin must be present within the tissue in order to maintain the enhancement, there is no relationship between the total amount of auxin and the accelerated Cl- uptake that results. A polarity in the auxin effect is implied since only apical applications of IAA promote Cl- uptake.
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Rubinstein, B., Light, E.N. Indoleacetic-acid-enhanced chloride uptake into coleoptile cells. Planta 110, 43–56 (1973). https://doi.org/10.1007/BF00386921
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DOI: https://doi.org/10.1007/BF00386921