Abstract
For valine uptake by the polychaete Nereis virens Sars, the kinetic constants were: V max=355 nmol g-1 fresh weight h-1, K m=20 μM. Leucine and some other amino acids acted as partial inhibitors of valine uptake. Valine uptake rate was 78% higher at 21.5‰ S than at 14‰ S. The major portion of valine absorbed by the polychaete could be extracted as free valine, with 6.5 to 15.6% being respired, and 3.6 to 9.5% incorporated into proteins. Calculations indicate that 7 to 12% of the metabolism of N. virens may be sustained by uptake of glycine and aspartic acid from natural concentrations. It is suggested that uptake of amino acids by this worm is important in the nitrogen cycling of marine sediments.
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Literature Cited
Anderson, J.W.: The uptake and incorporation of glycine by the gills of Rangia cuneata (Mollusca: Bivalvia) in response to variations in salinity and sodium. In: Physiological ecology of estuarine organisms, pp 239–258. Ed. by F. J. Vernberg. Columbia, South Carolina: University of South Carolina Press 1975
— and W.B. Bedford: The physiological response of the estuarine clam Rangia cuneata (Gray), to salinity. II. Uptake of glycine. Biol. Bull. mar. biol. Lab., Woods Hole 144, 229–247 (1973)
Bamford, D.R. and R. McCrea: Active absorption of neutral and basic amino acids by the gills of the common cockle, Cerastoderma edule. Comp. Biochem. Physiol. 50, 811–817 (1975)
Burgh, M.E., de: Specificity of L-alanine transport in the spine epithelium of Paracentrotus lividus (Echinoidea). J. mar. biol. Ass. U.K. 58, 425–440 (1978)
—, A.B. West and F. Jeal: Absorption of L-alanine and other dissolved nutrients by the spines of Paracentrotus lividus (Echinoidea). J. mar. biol. Ass. U.K. 57, 1031–1045 (1977)
Chien, P.K., G.C. Stephens and P.L. Healey: The role of ultrastructure and physiological differentiation of epithelia in amino acid uptake by the bloodworm, Glycera. Biol. Bull. mar. biol. Lab., Woods Hole 142, 219–234 (1972)
Crawford, C.C., J.E. Hobbie and K.L. Webb: The utilization of dissolved free amino acids by estuarine micoorganisms. Ecology 55, 551–563 (1974)
Ferguson, F.C.: Uptake and release of free amino acids by starfishes. Biol. Bull. mar. biol. Lab., Woods Hole 141, 122–129 (1971)
Gilles, R.: Mechanisms of ion and osmoregulation. In: Marine ecology. Vol. II. Physiological mechanisms, Part 1. pp 259–347. Ed. by O. Kinne. London: Wiley-Interscience 1975
Hanson, R.B. and W.S. Gardner: Uptake and metabolism of two amino acids by anaerobic microorganisms in four diverse salt-marsh soils. Mar. Biol. 46, 101–107 (1978)
Jørgensen, C.B.: August Pütter, August Krogh, and modern ideas on the use of dissolved organic matter in aquatic environments. Biol. Rev. 51, 291–328 (1976)
Jørgensen, N.O.G.: Annual variation of dissolved free primary amines in estuarine water and sediment. Oecologia (Berl.). (In press)
Lehninger, A.L.: Biochemistry, 833 pp. New York: Worth Publishers, Inc. 1970
Lopez, G.R. and J.S. Levinton: The availability of microorganisms attached to sediment particles as food for Hydrobia ventrosa Montagu (Gastropoda: Prosobranchia). Oecologia (Berl.) 32, 263–275 (1978)
Muus, B.: The fauna of Danish estuaries and lagoons. Meddr Danm. Fisk.-og Havunders. 5, 1–316 (1967)
Siebers, D.: Absorption of neutral and basic amino acids across the body surface of two annelid species. Helgoländer wiss. Meeresunters. 28, 456–466 (1976)
Southward, A.J. and E.C. Southward: Observations on the role of dissolved organic compounds in the nutrition of benthic invertebrates. II. Uptake by other animals living in the same habitat as pogonophores, and by some littoral polychaetes. Sarsia 48, 61–70 (1972)
Stephens, G.C.: Uptake of organic material by aquatic invertebrates. II. Accumulation of amino acids by the bamboo worm, Clymenella torquata. Comp. Biochem. Physiol. 10, 191–202 (1963)
—: Uptake of organic material by aquatic invertebrates. III. Uptake of glycine by brackish water annelids. Biol. Bull. mar. biol. Lab., Woods Hole 126, 150–162 (1964)
— Dissolved organic matter as a potential source of nutrition for marine organisms. Am. Zool. 8, 95–106 (1968)
—: Amino acid accumulation and assimilation in marine organisms. In: Nitrogen metabolism and the environment, pp 155–184. Ed. by J.W. Cambell and L. Goldstein. London: Academic Press 1972
—: Uptake of naturally occurring primary amines by marine annelids. Biol. Bull. mar. biol. Lab., Woods Hole 149, 397–407 (1975)
— and R.A. Virkar: Uptake of organic material by aquatic invertebrates. IV. The influence of salinity on the uptake of amino acids by the brittle star, Ophiactis arenosa. Biol. Bull. mar. biol. Lab., Woods Hole 131, 172–185 (1966)
Taylor, A.G.: The direct uptake of amino acids and other small molecules from sea water by Nereis virens Sars. Comp. Biochem. Physiol. 29, 243–250 (1969)
West, B., M. de Burgh and F. Jeal: Dissolved organics in the nutrition of benthic invertebrates. In: Biology of benthic organisms, pp 587–594. Ed. by B.F. Keegan, P.O. Ceidigh and P.J.S. Boaden. Oxford: Pergamon Press 1977
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Communicated by T.M. Fenchel, Aarhus
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Jørgensen, N.O.G. Uptake of L-valine and other amino acids by the polychaete Nereis virens . Mar. Biol. 52, 45–52 (1979). https://doi.org/10.1007/BF00386856
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DOI: https://doi.org/10.1007/BF00386856