Summary
After a prolonged culture on Murashige-Skoog medium the primary explants of hypocotyls and cotyledons of the pumpkin develop embryoid-producing callus tissue. Ten separate strains of such tissue have been obtained and have now been in culture for more than one year, continuing to produce embryoids.
References
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Jelaska, S. Embryoid formation by fragments of cotyledons and hypocotyls in Cucurbita pepo . Planta 103, 278–280 (1972). https://doi.org/10.1007/BF00386851
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DOI: https://doi.org/10.1007/BF00386851