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Glucosyltransferase activity in a water extract of maize pollen

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Summary

Glucosyltransferase activity was extracted from maize pollen in distilled water. The enzymatic reaction required UDP-glucose, mercaptoethanol and Ca++, and had a pH optimum at 8.2. Either kampferol or quercetin served as a substrate. Michaelis-Menten constants obtained were 0.6×10-4 M for quercetin and 0.74×10-3M for UDP-glucose. Ammonium-sulfate precipitation of the enzyme gave a 4fold purification.

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Author notes

  1. Cynthia M. Lonergan

    Present address: University of Missouri, Columbia, USA

Authors and Affiliations

  1. Department of Agronomy, University of Missouri, Columbia

    R. L. Larson & Cynthia M. Lonergan

  2. Department of Agriculture, University of Missouri, 304 Curtis Hall, 65201, Columbia, MO, USA

    R. L. Larson

Authors
  1. R. L. Larson
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  2. Cynthia M. Lonergan
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Additional information

Cooperative investigations, Plant Science Research Division, Agricultural Research Service, U. S. Department of Agriculture, and Missouri Agricultural Experiment Station, Columbia. Journal Series No. 6224.

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Larson, R.L., Lonergan, C.M. Glucosyltransferase activity in a water extract of maize pollen. Planta 103, 361–364 (1972). https://doi.org/10.1007/BF00386709

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  • DOI: https://doi.org/10.1007/BF00386709

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