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Pyrenoid protein from the brown alga Pilayella littoralis

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Abstract

Characterization by peptide mapping and amino acid analysis of the two major pyrenoid polypeptides from the brown alga Pilayella littoralis shows that they are very similar to the subunits of ribulose-1,5-bisphosphate carboxylase (EC 4.1.1.39) from this alga. The observed similarities are discussed in relation to previous pyrenoid protein characterization from members of the Chlorophyceae.

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Abbreviations

DTT:

dithiothreitol

EDTA Na2 :

ethylenediamine tetraacetic acid (disodium salt)

PMFS:

phenylmethylsul-phonylfluoride

PVPP:

polyvinylpyrrolidone

RuBP:

ribulose-1,5-bisphosphate

RuBPCase:

ribulose-1,5-bisphosphate carboxylase

SDS:

sodium dodecyl sulphate

SDS-PAGE:

sodium dodecyl sulphate polyacrylamide gel electrophoresis

TRIS:

2-amino-2-(hydroxymethyl) propane-1,3-diol

TPCK:

L-1-tosylamido-2-phenylethylchoromethyl ketone

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Authors and Affiliations

  1. Department of Plant Sciences, The University, LS2 9JT, Leeds, U.K.

    N. W. Kerby & L. V. Evans

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  1. N. W. Kerby
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  2. L. V. Evans
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Kerby, N.W., Evans, L.V. Pyrenoid protein from the brown alga Pilayella littoralis . Planta 151, 469–475 (1981). https://doi.org/10.1007/BF00386541

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  • DOI: https://doi.org/10.1007/BF00386541

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