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In-vitro auxin binding to particulate cell fractions from corn coleoptiles

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Summary

When low concentrations (e.g. 10-6 M) of labelled 3-indoleacetic acid (14C-IAA) or α-naphthaleneacetic acid (14C-NAA) are added in vitro to homogenates of corn coleoptiles, radioactivity is reversibly bound to pelletable particles. From the saturation kinetics of the binding it is possible to estimate an apparent K M between 10-6 M and 10-5 M and a concentration of specific sites of 10-7–10-6 M per tissue volume.

The binding is auxin-specific. Among many compounds tested, only auxins and such auxin analogues that are known to interact directly with auxin in transport and/or growth were found to interfere with this binding. For instance, the growth-active d-dichlorophenoxyisopropionic acid at 10-4 M inhibits 14C-NAA binding more than the less active l-isomer.

The auxin-binding fractions are practically free of DNA and cytochrome-C oxidase and contain binding sites for 1-naphthylphthalamic acid. The results are discussed in context with the hyothesis—derived mainly from physiological data—that auxin receptors are localized at the plasma membrane.

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References

  • Appelmans, F., Wattiaux, R. R., De Duve, C.: Tissue fractionation studies. 5. The association of acid phosphatase with a special class of cytoplasmic granules in rat liver. Biochem. J. 59, 438–445 (1955).

    Google Scholar 

  • Burton, K.: A study of the conditions and mechanism of diphenylamine reaction for the colorimetric estimation of DNA. Biochem. J. 62, 315 (1956).

    Google Scholar 

  • Cuatrecasas, P.: Insulin-receptor interactions in adipose tissue cells: Direct measurement and properties. Proc. nat. Acad. Sci. (Wash.) 68, 1264–1268 (1971).

    Google Scholar 

  • Dunnick, J. K., Marinetti, G. V.: Hormone action at the plasmamembrane level III. Epinephrine interaction with the rat liver plasmamembrane. Biochim. biophys. Acta (Amst.) 249, 122–134 (1971).

    Google Scholar 

  • Hertel, R., Evans, M. L., Leopold, A. C., Sell, H. M.: The specificity of the auxin transport system. Planta (Berl.) 85, 238–249 (1969).

    Google Scholar 

  • Hertel, R., Flory, R.: Auxin movement in corn coleoptiles. Planta (Berl.) 82, 123–144 (1968).

    Google Scholar 

  • Hertel, R., Leopold, A. C.: Versuche zur Analyse des Auxintransports in der Koleoptile von Zea mays L. Planta (Berl.) 59, 535–562 (1963).

    Google Scholar 

  • Housley, S.: Kinetics of auxin-induced growth. In: Handbuch der Pflanzenphysiologie (W. Ruhland, ed.), vol. XIV, p. 1007–1043 (1961).

  • Joensson, A.: Chemical structure and growth activity of auxins and anti-auxins. In: Handbuch der Pflanzenphysiologie. (W. Ruhland, ed.), vol. VIX, p. 959–1006 (1961).

  • Kasai, M., Changeux, J. P.: In vitro excitation of purified membrane fragments. III. Comparison of the dose-response curves to decamethonium with the corresponding binding curves of decamethonium to the cholinergic receptor. J. Membrane Biol. 6, 1–23 (1971).

    Google Scholar 

  • Lembi, C. A., Morré, D. J., Thomson, K.-St., Hertel, R.: N-1-Naphthylphthalamicacid-binding of a plasma membrane-rich fraction from maize coleoptiles. Planta (Berl.) 99, 37–45 (1971).

    Google Scholar 

  • Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951).

    PubMed  Google Scholar 

  • Tanada, T.: Antagonism between indoleacetic acid and abscisic acid on a rapid phytochrome-mediated process. Nature (Lond.) 236, 460–461 (1972)

    Google Scholar 

  • Thomson, K.-St.: The binding of N-1-naphthylphthalamic acid (NPA), an inhibitor of auxin transport, to particulate fractions of corn coleoptiles. In: Modern aspects of hormonal regulation in plant growth and development (Kaldewey, H. ed.). Weinheim: Verlag Chemie 1971 (in press).

    Google Scholar 

  • VanDerWoude, W. J., Lembi, C. A., Morré, D. J.: Auxin (2,4-D) stimulation (in vivo and in vitro) of polysaccharide synthesis in plasma membrane fragments isolated from onion stems. Biochem. biophys. Res. Commun. 46, 245–253 (1972).

    Google Scholar 

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Authors and Affiliations

  1. Institut für Biologie III, Universität Freiburg i.Br., Germany

    R. Hertel, K. -St. Thomson & V. E. A. Russo

  2. Department of Horticulture, Purdue University, 47907, Lafayette, IN, USA

    K. -St. Thomson

  3. Max-Planck-Institut für Molekulare Genetik, Ihnestraße 63-73, D-1000, Berlin 33, Germany

    V. E. A. Russo

Authors
  1. R. Hertel
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  2. K. -St. Thomson
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  3. V. E. A. Russo
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Hertel, R., Thomson, K.S. & Russo, V.E.A. In-vitro auxin binding to particulate cell fractions from corn coleoptiles. Planta 107, 325–340 (1972). https://doi.org/10.1007/BF00386394

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  • DOI: https://doi.org/10.1007/BF00386394

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