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In-vitro auxin binding to particulate cell fractions from corn coleoptiles

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Summary

When low concentrations (e.g. 10-6 M) of labelled 3-indoleacetic acid (14C-IAA) or α-naphthaleneacetic acid (14C-NAA) are added in vitro to homogenates of corn coleoptiles, radioactivity is reversibly bound to pelletable particles. From the saturation kinetics of the binding it is possible to estimate an apparent K M between 10-6 M and 10-5 M and a concentration of specific sites of 10-7–10-6 M per tissue volume.

The binding is auxin-specific. Among many compounds tested, only auxins and such auxin analogues that are known to interact directly with auxin in transport and/or growth were found to interfere with this binding. For instance, the growth-active d-dichlorophenoxyisopropionic acid at 10-4 M inhibits 14C-NAA binding more than the less active l-isomer.

The auxin-binding fractions are practically free of DNA and cytochrome-C oxidase and contain binding sites for 1-naphthylphthalamic acid. The results are discussed in context with the hyothesis—derived mainly from physiological data—that auxin receptors are localized at the plasma membrane.

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Authors and Affiliations

  1. Institut für Biologie III, Universität Freiburg i.Br., Germany

    R. Hertel, K. -St. Thomson & V. E. A. Russo

  2. Department of Horticulture, Purdue University, 47907, Lafayette, IN, USA

    K. -St. Thomson

  3. Max-Planck-Institut für Molekulare Genetik, Ihnestraße 63-73, D-1000, Berlin 33, Germany

    V. E. A. Russo

Authors
  1. R. Hertel
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  2. K. -St. Thomson
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  3. V. E. A. Russo
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Hertel, R., Thomson, K.S. & Russo, V.E.A. In-vitro auxin binding to particulate cell fractions from corn coleoptiles. Planta 107, 325–340 (1972). https://doi.org/10.1007/BF00386394

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  • DOI: https://doi.org/10.1007/BF00386394

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