Summary
When barley aleurone layers were incubated with 3H-Gibberellin A1 (3H-GA1), the hormone was converted to 3H-GA-X (not identified), 3H-GA8 and two other compounds tentatively identified as 3H-GA1-glucoside, and 3H-GA8-glucoside. Uptake and metabolism of the 3H-GA1 were markedly enhanced by simultaneous treatment with abscisic acid (ABA). Uptake of 3H-GA1 from the medium containing ABA was linear over a 24-h period, whereas in the absence of ABA, uptake of 3H-GA1 leveled off after 5 h. After 24 h, aleurones treated with 3H-GA1 and 3H-GA1 plus ABA, had taken up 9 and 24%, respectively, of the original 3H-GA1 provided. Metabolism of 3H-GA1 proceeded at a linear rate in the presence of ABA. The amount of 3H-GA1-metabolites which had accumulated by the end of a 24-h incubation appeared to be linearly correlated to the logarithm of the ABA concentration. Gibberellins A8 and-A8-glucoside did not reverse GA1-enhanced synthesis of α-amylase.
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References
Addicott, F. T., Lyon, J. L.: Physiology of abscisic acid and related substances. Ann. Rev. Plant Physiol. 20, 139–164 (1969).
Bray, G. A.: A simple, efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Analyt. Biochem. 1, 279–285 (1960).
Cherry, J. H.: Regulation of invertase activity in washed sugar beet tissue. In: Biochemistry and physiology of plant growth substances (F. Wightman, G. Setterfield, eds.), p. 417–431. Ottawa: Runge Press 1968.
Chrispeels, M. J., Varner, J. E.: Hormonal control of enzyme synthesis: On the mode of action of gibberellic acid and abscisin in aleurone layers of barley. Plant Physiol. 42, 1008–1016 (1967).
Crozier, A., Kuo, C. C., Durley, R. C., Pharis, R. D.: The biological activity of 26 gibberellins in nine bioassays. Canad. J. Bot. 48, 867–877 (1970).
Ginzburg, C., Kende, H.: Studies on the intracellular localization of radioactive gibberellin. In: Biochemistry and physiology of plant growth substances (F. Wightman, G. Setterfield, eds.), p. 333–340. Ottawa: Runge Press 1968.
Glew, R. H.: Developmental aspects of lipid metabolism in the developing and germinating castor bean seed. Doctoral dissert., University of California, Davis (1968).
Hemberg, T.: Abscisin II as an inhibitor of α-amylase. Acta chem. scand. 21, 1665–1666 (1967).
Jones, R. L.: Gibberellic acid enhanced release of β-1,3-glucanase from barley aleurone cells. Plant Physiol. 47, 412–416 (1971).
Jones, R. L., Varner, J. E.: The bioassay of gibberellins. Planta (Berl.) 72, 155–161 (1967).
Kende, H.: Preparation of radioactive GA1 and its metabolism in dwarf peas. Plant Physiol. 42, 1612–1618 (1967).
Lang, A.: Gibberellins: structure and metabolism. Ann. Rev. Plant Physiol. 21, 537–570 (1970).
Musgrave, A., Kays, S. E., Kende, H.: Uptake and metabolism of radioactive gibberellins by barley aleurone layers. Planta (Berl.) 102, 1–10 (1972).
Nadeau, R., Rappaport, L.: Metabolism of gibberellin A1 in germinating bean seeds. Phytochem. 11, 1611–1616 (1972).
Overbeek, J. van, Loeffler, J. E., Mason, M. I. R.: Mode of action of abscisic acid. In: Biochemistry and physiology of plant growth substances (F. Wightman, G. Setterfield, eds.), p. 1593–1607. Ottawa: Runge Press 1968.
Paleg, L., Aspinall, D., Coombe, B., Nicholls, P.: Physiological effects of gibberellic acid. VI. Other gibberellins in three test systems. Plant Physiol. 39, 286–290 (1964).
Pollard, C. J.: Initiation of responses in aleurone layers by gibberellic acid. Biochim. biophys. Acta (Amst.) 222, 501–507 (1970).
Schreiber, K., Weiland, J., Sembdner, G.: Isolierung von Gibberellin-A8-0(3)-β-D-glucopyranosid aus Früchten von Phaseolus coccineus. Phytochem. 9, 189–198 (1970).
Shih, C. Y., Rappaport, L.: Regulation of bud rest in tubers of potato, Solanum tuberosum L. VII. Effects of abscisic and gibberellic acids on nucleic acid synthesis in excised buds. Plant Physiol. 45, 33–36 (1970).
Smith, O. E., Sadri, H. A.: Effect of abscisic acid on gibberellin in biosynthesis in Fusarium moniliforme. Plant Cell Physiol. 11, 345–348 (1970).
Walton, D. C., Sondheimer, E.: Effects of abscisin II on phenylalanine ammonialyase activity in excised bean axes. Plant Physiol. 43, 467–469 (1968).
Wareing, P.F., Good, J., Manuel, J.: Some possible physiological roles of abscisic acid. In: Biochemistry and physiology of plant growth substances (F. Wightman, G. Setterfield, eds.), p. 1547–1560. Ottawa: Runge Press 1968.
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Nadeau, R., Rappaport, L. & Stolp, C.F. Uptake and metabolism of 3H-Gibberellin A1 by barley aleurone layers: Response to abscisic acid. Planta 107, 315–324 (1972). https://doi.org/10.1007/BF00386393
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DOI: https://doi.org/10.1007/BF00386393