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The use of nurse culture in the development of haploid clones in tomato

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Summary

A nurse culture procedure and an appropriate medium are described for obtaining vigorous haploid clones originating from single tomato pollen grains. The grains are plated on filter-paper disks mounted over intact anthers placed on a modified Murashige and Skoog medium. The plating efficiencies of such pollen cells ranges between 0 and 60%. The variability in the plating efficiencies and the potential use of this technique over that of anther culture are discussed

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Authors and Affiliations

  1. Department of Microbiology, Ohio State University, Columbus, USA

    W. R. Sharp & R. S. Raskin

  2. Department of Biochemistry, Ohio State University, Columbus, USA

    H. E. Sommer

Authors
  1. W. R. Sharp
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  2. R. S. Raskin
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  3. H. E. Sommer
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Additional information

Supported by Grants from the Commission Interamericana de Energia Nuclear and the College of Biological Sciences, Ohio State University.

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Sharp, W.R., Raskin, R.S. & Sommer, H.E. The use of nurse culture in the development of haploid clones in tomato. Planta 104, 357–361 (1972). https://doi.org/10.1007/BF00386318

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  • DOI: https://doi.org/10.1007/BF00386318

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