Abstract
Tuna condensate was a better substrate than shrimp-blanching water or effluent from a frozen-seafood plant for growing Rhodocyclus gelatinosus under anaerobic conditions in the light. One strain out of four examined, R7, gave the highest biomass (4.0 g/l), cell yield (0.32 g cell/g COD), and COD removal (78%) in 1:10 (v/v) diluted tuna condensate. Shrimp-blanching water added to the tuna condensate further increased growth rate, biomass and COD removal. Optimal growth was at pH 7.0 and 3000 Lux light intensity. Acetate, pyruvate, glucose, glutamate, propionate or malate added to the tuna condensate did not increase cell yield, carotenoid or bacteriochlorophyll content or biomass protein. A maximum cell mass of 5.6 g/l (containing 50% protein) and 86% COD removal were obtained after 5 days' incubation under optimal conditions.
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References
American Public Health Association 1985 Standard Methods for the Examination of Water and Wastewater, 16th edn. Washington DC: APHA.
Buchanan, R.E., Gibbons, N.E., Cowan, S.T., Holt, J.G., Liston, J., Murray, R.G.E. & Stanier, R.Y. (eds) 1974 Bergey's Manual of Determinative Bacteriology, 8th edn. Baltimore: Williams & Wilkins.
Devlin, R.M. & Barker, A.V. 1971 Photosynthesis. New Delhi: Affiliated East-West Press PVT.
Gest, H. 1961 Metabolic patterns in photosynthetic bacteria. Bacteriological Reviews 51, 183–209.
Hirayama, O. 1968 Lipids and lipoprotein complex in photosynthetic tissue: four lipids and pigments of photosynthetic bacteria. Agricultural and Biological Chemistry Journal 32, 34–41.
Jung, S., Prasertsan, P. & Buckle, K.A. 1990 Fish cannery wastewater characteristics. ASEAN Food Journal 5, 82–83.
Kerven, G. 1980 Applications of Atomic Absorption Spectroscopy to the Analysis of Biological Materials. University of Queensland, Brisbane: Department of Agriculture.
Kobayashi, M. 1977 Utilization and disposal of wastes by photosynthetic bacteria. In Microbial Energy Conversion, eds Schlegel, H.G. & Barnea, J. pp. 443–453. Oxford: Pergamon Press.
Kobayashi, M. & Kurata, S. 1978 The mass culture and cell utilization of photosynthetic bacteria. Process Biochemistry 13, 27–30.
Kobayashi, M., Shiomi, N. & Okuda, A. 1966 Studies on utilization of photosynthetic bacteria. Journal of Science Soil and Manure, Tokyo 37, 305–310.
Pfennig, N. 1967 Photosynthetic bacteria. Annual Review of Microbiology 21, 285–324.
Prasertsan, P. & Choorit, W. 1988 Problem and solution of the occurrence of red colour in wastewater of seafood processing plant. Songklanakarin Journal of Science and Technology 10, 439–446.
Prasertsan, P., Choorit, W. & Suwanno, S. 1993 Isolation, identification and growth condition of photosynthetic bacteria found in seafood processing wastewater. Would Journal of Microbiology and Biotechnology 9, 590–592.
Sasaki, K., & Nagai, S. 1979 The optimum pH and temperature for the aerobic growth of Rhodopseudomonas gelatinosa and vitamin B12 and ubiquinone formation on a starch medium. Journal of Fermentation Technology 57, 383–386.
Sasaki, K., Noparatnaraporn, N., Hayashi, M., Nshizawa, Y. & Nagai, S. 1981 Single-cell protein production by treatment of soybean wastes with Rhodopseudomonas gelatinosa. Journal of Fermentation Technology 59, 471–477.
Sawada, H. & Rogers, P.L. 1977 Photosynthetic bacteria in waste treatment: pure culture studies with Rhodopseudomonas capsulata. Journal of Fermentation Technology 55, 297–310.
VanNiel, C.B. 1954 The chemotrophic and photosynthetic bacteria. Annual Review of Microbiology 8, 105–132.
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Prasertsan, P., Choorit, W. & Suwanno, S. Optimization for growth of Rhodocyclus gelatinosus in seafood processing effluents. World J Microbiol Biotechnol 9, 593–596 (1993). https://doi.org/10.1007/BF00386302
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DOI: https://doi.org/10.1007/BF00386302