Abstract
A rapid and sensitive method for the detection of wild poliovirus from sewage samples using the polymerase chain reaction (PCR) technique was investigated. To eliminate the toxicity of sample concentrates to the enzymatic system used in PCR, a methodology was developed for the purification of these concentrates, consisting of treatment with trichlorofluoroethane and Sephadex column chromatography. The viral RNA was extracted from the purified concentrates, submitted to PCR with primers specific for Brazilian wild poliovirus type 1 and for Sabin types 1, 2 and 3. The amplified products were detected by electrophoresis in vertical polyacrylamide gels and stained with ethidium bromide. The results suggest that sewage sampling for environmental surveillance, combined with the rapid and precise PCR technology, provides a powerful tool for assessment of the success of the poliovirus eradication programme.
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References
American Public Health Association 1989 Standard Methods for the Examination of Water and Wastewater, 17th edn. Washington DC: American Public Health Association, American Water Works Association and Water Pollution Control Federation.
Berg, G., Bodily, H.L., Lennette, E.H., Melnick, J.L. & Metcalf, T.G. 1976 Viruses in Water. Washington DC: American Public Health Association.
Bitton, G., Farrah, S.R., Montague, C., Binford, M.W., Scheuerman, P.R. & Watson, A. 1984 Survey of Virus Isolation Data from Environmental Samples. Cincinnati, Ohio: US Environmental Protection Agency.
Bloom, H.H., Mack, W.N., Krueger, B.J. & Mallmann, W.L. 1959 Identification of enteroviruses in sewage. Journal of Infectious Diseases 105, 61–68.
Böttiger, M. 1973 Experiences from investigation of virus isolations from sewage over a two years period with special regard to poliovirus. Archiv für die Gesamte Virusforschung 41, 80–85.
De Leon, R. 1989 Use of genes probes and an amplification method for the detection of rotaviruses in water. PhD Thesis. University of Arizona, Tucson.
DeQuadros, C., Andrus, J.K., Olivé, J.M., Silveira, C.M., Eikhof, R.M., Carrasco, P., Fitzsimmons, J.W. & Pinheiro, F.P. 1991 Eradication of poliomyelitis: progress in the Americas. Pediatric Infectious Disease Journal 10, 222–229.
DaSilva, E.E., Pallansch, M.A., Holloway, B.P., Cuoto Oliveira, M.J., Schatzmayr, H.G. & Kew, O.M. 1990a Oligonucleotide probes for the specific detection of the wild poliovirus types 1 and 3 endemic to Brazil. Intervirology 32, 149–159.
DaSilva, E.E., Schatzmayr, H.G. & Kew, O.M. 1990b Nucleotide sequences of the VP1 capsid proteins of wild poliovirus types 1 and 3 from epidemic areas of Brazil. Brazilian Journal of Medical and Biological Research 22, 1–5.
DaSilva, E.E., Yang, C.-F., Filippis, A.M.B., De, L., Schatzmayr, H.G. & Kew, O.M. 1990c Use of the polymerase chain reaction for direct detection of wild polioviruses endemic to Brazil. In Abstract. VIIIth International Congress of Virology p. 345. Berlin: International Union of Microbiological Societies.
Erlich, H.A. 1989 PCR Technology: Principles and Applications for DNA Amplification. New York: Stockton Press.
Gerba, C.P., Margolin, A.B. & Hewlett, M.J. 1989 Application of gene probes to virus detection in water. Water Science and Technology 21, 147–154.
Horstmann, D.M., Emmons, J., Gimpel, L., Subrahmanyan, T. & Riordan, J.T. 1973 Enterovirus surveillance following a community-wide oral poliovirus vaccination program: a seven-year study. American Journal of Epidemiology 97, 173–186.
Hurst, C.J., Benton, W.H. & McClellan, K.A. 1989 Thermal and water source effects upon the stability of enteroviruses in surface freshwaters. Canadian Journal of Microbiology 35, 474–480.
Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. 1990 PCR Protocols: A Guide to Methods and Applications. San Diego: Academic Press.
Lund, E., Hedström, C.E. & Strannegard, Ö. 1966 A comparison between virus isolations from sewage and from fecal specimens from patients. American Journal of Epidemiology 84, 282–286.
Marques, E., Santos, V.M., Martinez, S.C.G.L. & Martins, M.T. 1993 Longitudinal study during massive oral poliovirus vaccination program to assess the circulation of wild poliovirus in sewage. In Abstracts, 1993 ASM Conference on Water Quality in the Western Hemisphere, p. 16. San Juan: American Society for Microbiology.
Martins, M.T., Marques, E. & Soares, L.A. 1982 A comparative study of methods for concentration of human enteric viruses from sewage. Water Science and Technology 14, 273–279.
Martins, M.T., Soares, L.A., Marques, E. & Molina, A.G. 1983 Human enteric viruses isolated from influents of sewage treatment plants in S. Paulo, Brazil. Water Science and Technology 15, 69–73.
Nelson, D.B., Circo, R. & Evans, A.S. 1967 Strategic viral surveillance of sewage during and following an oral poliovirus vaccine campaign. American Journal of Epidemiology 86, 641–652.
Pan American Health Organization 1985 Director announces campaign to eradicate poliomyelitis from the Americas by 1990. Bulletin of the World Health Organization 19, 213–215.
Pöyry, T., Stenvik, M. & Hovi, T. 1988 Viruses in sewage waters during and after a poliomyelitis outbreak and subsequent nationwide oral poliovirus vaccination campaign in Finland. Applied and Environmental Microbiology 54, 371–374.
Riordan, J.T. 1962 Isolation of enteroviruses from sewage before and after vaccine administration. Yale Journal of Biology and Medicine 34, 512–521.
Risi, J.B.Jr 1984 The control of poliomyelitis in Brazil. Reviews of Infectious Diseases 6, S400–403.
Sambrook, J., Fritsch, E.F. & Maniatis, T. 1989 Molecular Cloning: A Laboratory Manual. New York: Cold Spring Harbor Laboratory Press.
Ward, R.L. 1978 Mechanism of poliovirus inactivation by ammonia. Journal of Virology 26, 299–305.
Wiley, J.S., Chin, T.D.Y., Gravelle, C.R. & Robinson, S. 1962 Enterovirus in sewage during a poliomyelitis epidemic. Journal of the Water Pollution Control Federation 34, 168–178.
Wilterdink, J.B., Weiland, H.T. & Verlinde, J.D. 1970 A longitudinal study on the significance of examination of sewage for the presence of poliovirus in the population. Archiv für die Gesamte Virusforschung 32, 82–90.
World Health Assembly 1988 Global Eradication of Poliomyelitis by the Year 2000. Resolution WHA 41.28. Geneva: World Health Organization.
Yang, C.-F., De, L., Holloway, B.P., Pallansch, M.A. & Kew, O.M. 1991 Detection and identification of vaccine-related polioviruses by the polymerase chain reaction. Virus Research 20, 159–179.
Yang, C.-F., De, L., Yang, S.-J., Gömez, J.R., Cruz, J.R., Holloway, B.P., Pallansch, M.A. & Kew, O.M. 1992 Genotype-specific in vitro amplification of sequences of the wild type 3 polioviruses from Mexico and Guatemala. Virus Research 24, 277–296.
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Marques, E., Da Silva, E.E., Dos Santos, V.M. et al. Application of the polymerase chain reaction (PCR) to poliomyelitis surveillance through the analyses of sewage samples. World J Microbiol Biotechnol 9, 566–569 (1993). https://doi.org/10.1007/BF00386295
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DOI: https://doi.org/10.1007/BF00386295