Summary
Radioactive uridine is incorporated by Chlorella strain 211-8b/p into ribosomal subunits and their rapidly labelled RNA comigrates with chloroplast RNA on polycrylamide gels.
Ribosomal particles which can be labelled by short pulses of orotic acid cosediment with the particles labelled by uridine pulses and contain the same RNA species as these when separated either on sucrose gradients or on polycrylamide gels. This incorporation is, like that of uridine, sensitive to rifampin and chloramphenicol, but insensitive to cycloheximide.
A comparative study of short-time incorporation of uridine, orotic acid and guanosine into the RNA of Chlorella showed that all three precursors were incorporated mainly into RNA of chloroplastic origin. However, guanosine was also partly incorporated into cytoplasmic rRNA. Nitrogen-deficient cells always incorporated part of all three precursors into cytoplasmic rRNA, but the proportions of these were different among the different precursors.
These results are consistent with the hypothesis that the described differences in the incorporation of the above mentioned precursors into RNA of different cellular compartments are largely attributable to effects of pool sizes.
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References
Bucher, N. L. R., Swaffield, M. N.: Ribonucleic acid synthesis in relation to precursor pools in regenerating rat liver. Biochim. biophys. Acta (Amst.) 174, 491–502 (1969).
Galling, G.: Der Einfluß von Rifampicin, Chloramphenicol und Cycloheximid auf den Uridin-Einbau in chloroplastidäre Ribosomenvorstufen von Chlorella. Planta (Berl.) 98, 50–62 (1971).
Galling, G.: Einbau von Uridin und Orotsäure in plastidäre ribosomale RNA von Chlorella nach Antibiotica-Behandlung. Arch. Mikrobiol. 81, 245–259 (1972).
Galling, G., Ssymank, V.: Bevorzugter Einbau markierten Uridins in die Vorläufer von Chloroplasten-Ribosomen in Algenzellen. Planta (Berl.) 94, 203–212 (1970).
Iwamura, T., Kanazawa, T., Kanazawa, K.: Nucleotide metabolism in Chlorella. In: Studies on microalgae and photosynthetic bacteria, p. 577–596. Jap. Soc. Plant Physiologists, ed. Tokyo: The University of Tokyo Press 1963.
Loening, U. E.: The fractionation of high molecular weight ribonucleic acid by polyacrylamide gel electrophoresis. Biochem. J. 102, 251–257 (1967).
Newmark, P., Fujimoto, Y.: Nucleic acids of nitrogen deficient Chlorella pyrenoidosa. Fed. Proc. 18, 293 (1959)
Parish, J. H., Kirby, K. S.: Reagents which reduce interactions between ribosomal RNA and rapidly labelled RNA from rat liver. Biochim. biophys. Acta (Amst.) 129, 554–562 (1966).
Ssymank, V.: Influence of nitrogen deficiency on uridine incorporation into ribosomes in the green alga Chlorella. Arch. Mikrobiol. 82, 311–324 (1972).
Ssymank, V., Galling, G.: Labelling of chloroplast ribosomes by uridine in Chlorella. Abstr. Commun. Meet. Fed. Eur. Biochem. Soc. 8, No 496 (1972).
Stambrook, P. J., Sisken, J. E.: The relationship between rates of 3H-uridine and 3H-adenine incorporation into RNA and the measured rates of RNA synthesis during the cell cycle. Biochim. biophys. Acta (Amst.) 281, 45–54 (1972).
Surzycki, S., Hastings, P. J.: Control of chloroplast RNA synthesis in Chlamydomonas reinhardii. Nature (Lond.) 220, 786–787 (1968).
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Ssymank, V. Incorporation of different labelled precursors into chloroplast RNA of Chlorella . Planta 111, 157–166 (1973). https://doi.org/10.1007/BF00386276
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DOI: https://doi.org/10.1007/BF00386276