Summary
Axes of Phaseolus vulgaris cease synthesis of RNA during the maturation stage of embryogeny. During the imbibition phase of germination RNA synthesis resumes after the axes reach a normal water content. In the first hour of inbibition a very low rate of incorporation of 3H-adenosine into is RNA found, and the primary site of incorporation is the-CCA end of tRNA. At later stages of germination tRNA end-labeling accounts for a minor fraction of adenosine incorporation. The rate of RNA synthesis increases after initiation of axis elongation to a maximal rate at 18 h of germination. ATP pool-size and specific activity vary over a several-fold range during development, an important consideration in determining the rate of RNA synthesis.
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Supported by a grant from the National Science Foundation (GB 8709) to M. E. Clutter and I. M. Sussex and by a National Science Foundation Predoctoral Fellowship (V. W.). Submitted to the Graduate School, Yale University in partial fulfillment of the requirements for the degree of Doctor of Philosophy.
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Walbot, V. Rate of RNA synthesis and tRNA end-labeling during early development of Phaseolus . Planta 108, 161–171 (1972). https://doi.org/10.1007/BF00386078
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DOI: https://doi.org/10.1007/BF00386078