Phytochrome-controlled rapid contraction and recovery of contractile vacuoles in the motor cells of Mimosa pudica as an intracellular correlate of nyctinasty

Summary

Using living thin sections (ca. 70–80 μ thick) of tertiary pulvini of Mimosa pudica, we have quantitatively determined that the bahavior of the contractile tannin vacuoles in the motor cells is under phytochrome control. Using material in which these vacuoles were in their most expanded state in white light, contraction was observable 3 min after the material was placed in continuous darkness. No contraction occurred if the cells were irradiated with 90 sec of far-red light; red light reversed this effect. Futhermore, the kinetics of change of the vacuolar conformation was closely paralled by that of the nyctinastic changes of the pinnule closure during the different treatments. When the section of pulvinus was irradiated with a microbeam of far red light in one part of the section, and the motor cell vacuoles in another area were monitored for contraction, they almost always responded.

We therefore conclude that the contractile vacuole of the motor cell is an excellent cellular correlate of phytochrome-mediated nyctinasty in M. pudica, and discuss its possible causal role in regulating the phenomenon. It is further concluded that functional phytochrome is present in all parts of the pulvinus and that, upon absorption of the stimulus energy, an intercellular messenger is released which stimulates all the motor cells in the pulvinus.