Abstract
When Lemna minor L. is transferred to an atmosphere with H2S, there is a rapid loss of extractable adenosine-5′-phosphosulfate sulfotransferase activity. The activity is restored within 24 h in an atmosphere without H2S. This restoration of activity is completely inhibited by cycloheximid but not by chloramphenicol. In vitro, S2- up to 5 mM and cysteine, methionine, and glutathione up to 50 mM do not inhibit the enzyme. The activities of ATP sulfurylase and O-acetyl-L-serine sulfhydrylase are not affected significantly by H2S. The physiological significance of the regulation of adenosine-5′-phosphosulfate sulfotransferase is discussed.
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Abbreviations
- APS:
-
adenosine-5′-phosphosulfate
- PAPS:
-
adenosine-3′-phosphate-5′-phosphosulfate
- BSA:
-
bovine serum albumin
- DTT:
-
dithiothreitol
- POPOP:
-
1,4-di [2-(5-phenyloxazolyl)]-benzene
- PPO:
-
2,5-diphenyloxazol
References
Brunold, Chr., Erismann, K.H.: H2S als Schwefelquelle bei Lemna minor L.: Einfluß auf das Wachstum, den Schwefelgehalt und die Sulfataufnahme. Experientia 30, 465–467 (1974)
Brunold, Chr., Erismann, K.H.: H2S as sulfur source in Lemna minor L. II. Direct incorporation into cysteine and inhibition of sulfate assimilation. Experientia 31, 508–509 (1975)
Erismann, K.H., Finger, A.: Lemnaceen in kontinuierlicher Kultur. Ber. Schweiz. Bot. Ges. 78, 5–15 (1968)
Erismann, K.H., Brunold, Chr.: Die Verwendung einer neuen Lemna-Kulturanlage in Wachstums- und Stoffwechseluntersuchungen mit gelösten und gasförmigen Schwefelverbindungen. Ber. Schweiz. Bot. Ges. 83, 213–222 (1973)
Ellis, J.R.: Sulphate activation in higher plants. Planta (Berl.) 88, 34–42 (1969)
Giovanelli, A., Mudd, S.H.: Sulfurylation of O-acetyl-homoserine and O-acetylserine by two enzyme fractions from spinach. Biochem. biophys. Res. Commun. 31, 275–280 (1968)
Hendrickson, H.R., Conn, E.E.: Cyanide metabolis in higher plants. IV. Purification and properties of the β-cyanoalanine synthase of blue lupine. J. Biol. Chem. 244, 2632–2640 (1969)
Hodson, R.C., Schiff, J.A.: Preparation of adenosine-3′-phosphate-5′-phosphosulfate (PAPS): An impvoved enzymatic method using Chlorella pyrenoidosa. Arch. Biochem. Biophys. 132, 151–156 (1969)
Kuchar, K.W.: Bakteriologische und limnologische Untersuchungen an einem Lemnagewässer. Arch. f. Hydrobiol. 49, 329–334 (1954)
Landolt, E.: Physiologische und ökologische Untersuchungen an Lemnaceen. Ber. Schweiz. Bot. Ges. 67, 271–410 (1957)
Loussaert, D.F.: A model of enzymatic nitrite reduction in corn (Zea mays L.). Ph. D. Thesis, Department of Agronomy, University of Illinois, USA 1975
Masada, M., Fukushima, K., Tamura, G.: Cysteine synthase from rape leaves. J. Biol. Chem. 77, 1107–1115 (1975)
Orebamjo, T.O., Stewart, G.R.: Ammonium repression of nitrate reductase formation in Lemna minor L. Planta (Berl.) 122, 27–36 (1975)
Pieniazek, N.J., Stephien, P.P., Pazewski, A.: An Aspergillus nidulans mutant lacking cystathionine β-synthase: Identity of L-serine sulfhydrolase with cystathionine β-synthase and its distinctness from O-acetyl-L-serine sulfhydrolase. Biochim. Biophys. Acta 297, 37–47 (1973)
Robbins, P.W.: Sulfate-activating enzymes. In: Methods in Enzymology, vol. 5, pp 964–977. Ed. Colowick, S.P. and Kaplan, N.O. New York and London: Academic Press 1962
Schiff, J.A., Hodson, R.C.: The metabolism of sulfate. Ann. Rev. Plant Physiol. 24, 381–414 (1973)
Schmidt, A., Abrams, W.R., Schiff, J.A.: Reduction of adenosine-5′-phosphosulfate to cysteine in extracts from Chlorella and mutants blocked for sulfate reduction. Europ. J. Biochem. 47, 423–434 (1974)
Schmidt, A.: Distribution of APS-sulfotransferase activity among higher plants. Plant Sci. Lett. 5, 407–415 (1975a)
Schmidt, A.: A sulfotransferase from spinach leaves using adenosine 5′-phosphosulfate. Planta (Berl.) 124, 267–275 (1975b)
Schmidt, A.: Development of the adenosine-5′-phosphosulfate sulfotransferase in sunflower (Helianthus annuus L.). Z. Pflanzenphys. 78, 164–168 (1976)
Thompson, J.F.: Sulfur metabolism in plants. Ann. Rev. Plant Physiol. 18, 59–84 (1967)
Thompson, J.F., Moore, D.P.: Enzymatic synthesis of cysteine and S-methylcysteine in plant extracts. Biochem. biophys. Res. Commun. 31, 281–286 (1968)
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This is no. 6 in the series “Regulation of Sulfate Assmilation in Plants”
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Brunold, C., Schmidt, A. Regulation of adenosine-5′-phosphosulfate sulfotransferase activity by H2S in Lemna minor L.. Planta 133, 85–88 (1976). https://doi.org/10.1007/BF00386010
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DOI: https://doi.org/10.1007/BF00386010