Summary
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1.
A typical growth curve including a lag phase, phases of cell division and cell expansion and a stationary phase could be demonstrated for batch propagated cells ofGlycine max. in synthetic liquid medium.
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2.
Shortly before the stationary phase was reached, dramatic changes in the activities of two enzymes of “secondary plant metabolism”, phenylalanine ammonia-lyase and p-coumarate: CoA ligase, were observed. In contrast, the activity of acetate:CoA ligase remained constant during this period of time, indicating that primary cell metabolism was unaffected.
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3.
Activities of both enzymes, phenylalanine ammonia-lyase and p-coumarate: CoA ligase, could be enhanced by light. Using disc gel electrophoresis it was not possible to detect isoenzymes of phenylalanine ammonia-lyase in extracts either from cells grown in the dark or from those treated with light.
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Hahlbrock, K., Kuhlen, E. & Lindl, T. Änderungen von Enzymaktivitäten während des Wachstums von Zellsuspensionskulturen vonGlycine max: Phenylalanin Ammonium-Lyase und p-Cumarat: CoA Ligase. Planta 99, 311–318 (1971). https://doi.org/10.1007/BF00385823
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DOI: https://doi.org/10.1007/BF00385823