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Metabolism of [13C1]gibberellin A29 to [13C1]gibberellin-catabolite in maturing seeds of Pisum sativum cv. Progress No. 9

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Abstract

The metabolism of GA29 during seed maturation in Pisum sativum cv. Progress No. 9 was further investigated. [17-13C1]GA29 was metabolised to a GA-catabolite (structure 3), with incorporation of the [13C] label from the GA29 substrate into the GA-catabolite being demonstrated by GC-MS. Quantitation of the GA-catabolite using GC-MS was achieved by adding GA-catabolite, labelled with [18O], to seed extracts as an internal standard. At least 50% conversion of [13C1]GA29 to [13C1]GA-catabolite was demonstrated with the build up of exogenous [13C1]GA-catabolite strictly paralleling the accumulation of native GA-catabolite. These results strongly suggest that conversion of GA29 to the GA-catabolite is a natural metabolic step occurring during the final stages of seed maturation. 25 μg per seed of native GA-catabolite was recorded in 37 day old seeds. Some problems encountered in the analysis of extracts containing the GA-catabolite are discussed briefly.

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Abbreviations

BSTFA:

bis(trifluoromethylsilyl)acetamide

GAn :

gibberellin An

GC:

gas chromatography

GC-MS:

combined gas chromatography-mass spectrometry

Me:

methyl ester

SICM:

selected ion current monitoring

TMSi:

trimethylsilyl ether

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Authors and Affiliations

  1. School of Chemistry, University of Bristol, BS8 1TS, Bristol, UK

    Valerie M. Sponsel & Jake MacMillan

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  1. Valerie M. Sponsel
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  2. Jake MacMillan
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Sponsel, V.M., MacMillan, J. Metabolism of [13C1]gibberellin A29 to [13C1]gibberellin-catabolite in maturing seeds of Pisum sativum cv. Progress No. 9. Planta 150, 46–52 (1980). https://doi.org/10.1007/BF00385614

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  • DOI: https://doi.org/10.1007/BF00385614

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