Abstract
The synchronized divisions following a treatment with hydroxyurea (HU) — an inhibitor of DNA synthesis — were studied in root meristems of Allium sativum using two methods: autoradiography of median sections and morphological labeling with a cytokinesis inhibitor. It is shown that the second wave of mitoses is heterogeneous: it is composed mostly of cells which have been synchronized in the S phase by the HU treatment, of cells coming from the quiescent center stimulated to enter DNA synthesis and of cells which were not blocked by the 23 h HU treatment (slow cycling cells). It is also shown that the cell cycle following the first synchronized division is considerably shortened by the synchronization procedure.
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Abbreviations
- QC:
-
quiescent center
- HU:
-
hydroxyurea
- MHQD:
-
methyl-3 hydroxy-6 quinazoline dione 2–4
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Clain, E., Brulfert, A. Hydroxyurea-induced mitotic synchronization in Allium sativum root meristems. Planta 150, 26–31 (1980). https://doi.org/10.1007/BF00385610
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DOI: https://doi.org/10.1007/BF00385610