Abstract
In vitro binding of 1-naphthaleneacetic acid (NAA) to particulate fractions from tobacco leaf protoplasts was studied. In freshly isolated protoplasts no specific binding could be detected, whereas it was present in particulate fractions from tobacco leaves. It is concluded that the NAA-binding-sites are probably located at the external face of the plasma membrane; they are destroyed during protoplast isolation by proteolytic enzymes in the cellulase and macerozyme preparations. After culturing the protoplasts for 3–4 d, the first cell divisions were observed and at the same time specific NAA-binding became detectable. The affinity constant for NAA was approx. 2·106 mol-1 and the number of binding sites increased during further culture.
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Abbreviations
- MES:
-
4-morpholinoethanesulfonic acid
- NAA:
-
1-naphthaleneacetic acid
References
Batt, S., Venis, M.A. (1976) Separation and localization of two classes of auxin binding-sites in corn coleoptiles. Planta 130, 15–21
Cocking, E.C. (1972) Plant cell protoplasts-isolation and development. Ann. Rev. Plant Physiol. 23, 29–50
Dohrmann, U., Hertel, R., Kowalik, H. (1978) Properties of auxin binding-sites in different subcellular fractions from maize coleoptiles. Planta 140, 97–106
Fleck, J., Durr, A., Lett, M.C., Hirth, L. (1979) Changes in protein synthesis during the initial stage of life of tobacco protoplasts. Planta 145, 279–285
Gamborg, O.L., Miller, R.A., Ojima, K. (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50, 151–158
Hendriks, T. (1978) The distribution of glucan synthetase in maize coleoptiles: a comparison with K-ATPase. Plant Sci. Lett. 11, 261–274
Hertel, R., Flory, R. (1968) Auxin movement in corn coleoptiles. Planta 82, 123–144
Hertel, R., Thomson, K.S., Russo, V.E.A. (1972) In-vitro auxin binding to particulate cell fractions from corn coleoptiles. Planta 107, 325–340
Kende, H., Gardner, G. (1976) Hormone binding in plants. Ann. Rev. Plant Physiol. 27, 267–290
Lázár, G., Borbély, G., Udvardy, J. Premecz, G., Farkas, G.L. (1973) Osmotic shock triggers an increase in ribonuclease level in protoplasts isolated from tobacco leaves. Plant Sci. Lett. 1, 53–57
Narahashi, Y. (1970) Pronase. In: Methods in enzymology, vol. XIX, pp. 651–664, Colowick, S.P., Kaplan, N.O., eds. Academic Press, New York London
Normand, G., Hartmann, M.A., Schuber, F., Benveniste, P. (1975) Charactérisation de membranes de coléoptiles de maïs fixant l'auxine et l'acide N-naphtyl phtalamique. Physiol. Vég. 13, 743–761
Oostrom, H., Loopik-Detmers, M.A. van, Libbenga, K.R. (1975) A high-affinity receptor for indoleacetic acid in cultured tobacco pith explants. FEBS Lett. 59, 194–197
Quail, P.H., Hughes, J.E. (1977) Phytochrome and phosphotungstate-chromate-positive vesicles from Cucurbita pepo L. Planta 133, 169–177
Racusen, R.H., Kinnersley, A.M., Galston, A.W. (1977) Osmotically induced changes in electrical properties of plant protoplast membranes. Science 198, 405–407
Ray, P.M. (1977) Auxin-binding-sites of maize coleoptiles are localized on membranes of the endoplasmic reticulum. Plant Physiol. 59, 594–599
Ray, P.M., Dohrmann, U., Hertel, R. (1977) Characterization of naphthaleneacetic acid binding to receptor sites on cellular membranes of maize coleoptile tissue. Plant Physiol. 59, 357–364
Ruesink, A. (1973) Surface membrane properties of isolated protoplasts. Colloq. Int. C.N.R.S. 212, 41–49
Silverstein, S.C., Steinman, R.M., Cohn, Z.A. (1977) Endocytosis. Annu. Rev. Biochem. 46, 669–722
Takebe, I., Nagata, T. (1973) Culture of isolated tobacco mesophyll protoplasts. Colloq. Int. C.N.R.S. 212, 175–188
Takebe, I., Otsuki, Y. (1973) Fine structure of isolated mesophyll protoplasts of tobacco. Planta 113, 21–27
Taylor, A.R.D., Hall, J.L. (1978) Fine structure and cytochemical properties of tobacco leaf protoplasts and comparison with the source tissue. Protoplasma 96, 113–126
Vreugdenhil, D., Burgers, A., Libbenga, K.R. (1979) A particle-bound auxin receptor from tobacco pith callus. Plant Sci. Lett. 16, 115–121
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Vreugdenhil, D., Harkes, P.A.A. & Libbenga, K.R. Auxin-binding by particulate fractions from tobacco leaf protoplasts. Planta 150, 9–12 (1980). https://doi.org/10.1007/BF00385607
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DOI: https://doi.org/10.1007/BF00385607