Summary
Several types of leaves of leaf parts lacking chlorophyll were fixed and embedded according to conventional procedures and examined electron-microscopically for microbodies. Comparisons of relative abundance of microbodies, plastids and mitochondria were made by computing the average numbers of organelle profiles per cell section. Similar leaves were homogenized and assayed for three enzymes characteristic of leaf peroxisomes. The localization of these enzymes in microbodies was indicated for the achlorophyllous tissues by the positive result obtained when 3,3′-diaminobenzidine was used as an electron cytochemical stain for catalase activity.
Microbodies were present in all non-photosynthetic leaves or leaf parts examined, including yellowish-white segments of variegated leaves, albino leaves, and etiolated leaves of two species. In several cases, the numbers of microbody profiles per cell section were as great in the achlorophyllous leaves as in the chlorophyllous. The levels of peroxisomal enzyme activity in the yellowish-white leaves were substantial, although often not as high as in the green leaves. It was concluded that enzymatically these microbodies are probably similar to the peroxisomes characterized from chlorophyllous leaves. In the absence of the photosynthetic product, glycolate, however, it seems unlikely that the organelle is performing the same functions as in green leaves. It is also apparent that the initial formation of peroxisomes in leaves can occur when neither light nor a photosynthate such as glycolate is present as an inducer.
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References
Appleman, D.: Catalase-chlorophyll relationship in barley seedlings. Plant Physiol. 27, 613–621 (1952).
Arnon, D. I.: Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol. 24, 1–15 (1949).
Duve, C. de: Functions of microbodies (peroxisomes). J. Cell Biol. 27, 25A-26A (1965).
Duve, C. de, Baudhuin, P.: Peroxisomes (microbodies and related particles). Physiol. Rev. 46, 323–357 (1966).
Eyster, H. C.: Catalase activity in chloroplast pigment deficient types of corn. Plant Physiol. 25, 630–638 (1950).
Frederick, S. E., Newcomb, E. H.: Microbody-like organelles in leaf cells. Science 163, 1353–1355 (1969a).
Frederick, S. E., Newcomb, E. H.: Cytochemical localization of catalase in leaf microbodies (peroxisomes). J. Cell Biol. 43, 343–353 (1969b).
Frederick, S. E., Newcomb, E. H.: Ultrastructure and distribution of microbodies in leaves of grasses with and without CO2-photorespiration. Planta (Berl.) 96, 152–174 (1971).
Gruber, P. J., Trelease, R. N., Becker, W. M., Newcomb, E. H.: A correlative ultrastructural and enzymatic study of cotyledonary microbodies following germination of fat-storing seeds. Planta (Berl.) 93, 269–288 (1970).
Kearney, P. C., Tolbert, N. E.: Appearance of glycolate and related products of photosynthesis outside of chloroplasts. Arch. Biochem. 98, 164–171 (1962).
Kisaki, T., Tolbert, N. E.: Glycolate and glyoxylate metabolism by isolated peroxisomes or chloroplasts. Plant Physiol. 44, 242–250 (1969).
Kuczmak, M., Tolbert, N. E.: Glycolic acid oxidase formation in greening leaves. Plant Physiol. 37, 729–734 (1962).
Linsmaier, E. M., Skoog, F.: Organic growth factor requirement of tobacco tissue cultures. Physiol. Plantarum (Cph.) 18, 100–127 (1965).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951).
Lück, H.: Catalase. In: Methods of enzymatic analysis, 885–894, H. U. Bergmeyer, ed. New York: Acad. Press 1963.
Marty, D.: Peroxysomes (=microbodies) associés aux plastes des zones non chlorophylliennes des feuilles panachées de Coleus blumei Benth. C. R. Acad. Sci. (Paris) 273, 860–863 (1971).
Newcomb, E. H., Frederick, S. E.: Distribution and structure of plant microbodies (peroxisomes). In: Photosynthesis and photorespiration, p. 442–457, M. D. Hatch, C. B. Osmond and R. O. Slatyer, eds. New York: Wiley-Interscience 1971.
Tamàs, I. A., Yemm, E. W., Bidwell, R. G. S.: The development of photosynthesis in dark-grown barley leaves upon illumination. Canad. J. Bot. 48, 2313–2317 (1970).
Tolbert, N. E.: Microbodies-peroxisomes and glyoxysomes. Ann. Rev. Plant Physiol. 22, 45–74 (1971a).
Tolbert, N. E.: Isolation of leaf peroxisomes. In: Methods in enzymology, vol. 23A, p. 665–682, A. San. Pietro, ed. New York: Acad. Press 1971b.
Tolbert, N. E., Burris, R. H.: Light activation of the plant enzyme which oxidizes glycolic acid. J. biol. Chem. 186, 791–804 (1950).
Tolbert, N. E., Cohan, M. S.: Activation of glycolic acid oxidase in plants. J. biol. Chem. 204, 639–648 (1953).
Tolbert, N. E., Oeser, A., Kisaki, T., Hageman, R. H., Yamazaki, R. K.: Peroxisomes from spinach leaves containing enzymes related to glycolate metabolism. J. biol. Chem. 243, 5179–5184 (1968).
Tolbert, N. E., Oeser, A., Yamazaki, R. K., Hageman, R. H., Kisaki, T.: A survey of plants for leaf peroxisomes. Plant Physiol. 44, 135–147 (1969).
Tolbert, N. E., Yamazaki, R. K.: Leaf peroxisomes and their relation to photorespiration and photosynthesis. Ann. N. Y. Acad. Sci. 168, 325–340 (1969).
Tolbert, N. E., Yamazaki, R. K., Oeser, A.: Localization and properties of hydroxypyruvate and glyoxylate reductases in spinach leaf particles. J. biol. Chem. 245, 5129–5136 (1970).
Trelease, R. N., Becker, W. M., Gruber, P. J., Newcomb, E. H.: Microbodies (glyoxysomes and peroxisomes) in cucumber cotyledons. Correlative biochemical and ultrastructural study in light- and darkgrown seedlings. Plant Physiol. 48, 461–475 (1971).
Zelitch, I.: The relationship of glycolic acid to respiration and photosynthesis in tobacco leaves. J. biol. Chem. 234, 3077–3081 (1959).
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Gruber, P.J., Becker, W.M. & Newcomb, E.H. The occurrence of microbodies and peroxisomal enzymes in achlorophyllous leaves. Planta 105, 114–138 (1972). https://doi.org/10.1007/BF00385572
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DOI: https://doi.org/10.1007/BF00385572