Abstract
Isolated embryos are more suitable for in vivo study of protein synthesis than non-isolated embryos because, after isolation, the uptake of labeled amino acids is about 1000 times higher. However, isolation also stimulates protein synthesis: Up to 4 h after isolation, the capacity to incorporate labeled amino acids increased 7 times. Therefore, data on incorporation obtained with isolated embryos cannot be extended to non-isolated embryos. The increase of protein synthesis was not due to synthesis of specific proteins, but was a general increase. Furthermore, ripening, dormant, and afterripened embryos showed the same degree of increase. Isolation therefore stimulates protein synthesis nonspecifically. When embryos were kept under anaerobic conditions after isolation, protein synthesis did not increase. Therefore, higher oxygen consumption after removal of the seedcoat is probably the cause of the higher incorporation capacity. Furthermore, the activation of protein synthesis lagged several hours behind the increase of oxygen consumption.
Similar content being viewed by others
Abbreviations
- A:
-
afterripened
- D:
-
dormant
- pre-rRNA:
-
precursor of ribosomal RNA
References
Bernhardt, D., Hecker, M., Bernhardt, H. (1978) Eignung intakter Caryophyllaceen Samen für die Bestimmung der DNA-, RNA-und Proteinsynthese durch Einbauversuche. Biochem. Physiol. Pflanzen 172, 263–270
Borriss, H. (1956) Über einige Ergebnisse und Probleme der Keimungsphysiologie. Wiss. Z. Univ. Greifswald, Math.-Nat. Reihe 6, 251–265
Grzelczak, Z., Buchowiez, J. (1977) A comparison of the activation of ribosomal RNA synthesis during germination of isolated and non-isolated embryos of Triticum aestivum L. Planta 134, 263–265
Günther, G., Borriss, H. (1967) Atmung und Endoxydasenaktivität der Samen von Agrostemma githago während der Nachreife. In: Physiologie, Ökologie und Biochemie der Keimung. pp. 527–533, Borriss, H., ed., Ernst-Moritz-Arndt-Universität, Greifswald
Hecker, M. (1976) Untersuchungen über Nucleinsäuren- und Proteinbiosynthesen während der Embryoentwicklung von Agrostemma githago. Biol. Zentralbl. 95: 25–33
Hecker, M., Köhler, K.-H., Wiedmann, M. (1977) Reactivation of ribonucleic acid synthesis during early germination of Agrostemma githago embryos. Biochem. Physiol. Pflanzen 171, 401–408
Klein, S., Negbi, M., Witztum, A., Rothberg, L. (1971) The role of the endosperm in uptake and distribution of exogeneous leucine in germinating lettuce seeds. New Phytol. 70, 143–147
de Klerk, G.J. (1981) Degradation of early synthesized proteins in imbibed dormant and afterripened Agrostemma githago L. embryos. Dev. Biol. 83:183–187
de Klerk, G.J., Linskens, H.F. (1979) Protein synthesis in dormant and afterripened Agrostemma githago L. embryos. Biochem. Physiol. Pflanzen 174, 537–545
Yemm, E.W. (1965) The respiration of plants and their organs. In: Plant physiology: a treatise, vol. IV A, pp. 231–310, Steward, F.C. ed., Academic Press, New York
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
de Klerk, G.J. Increase of protein synthesis after isolation in dormant and afterripened Agrostemma githago L. embryos. Planta 153, 524–529 (1981). https://doi.org/10.1007/BF00385536
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00385536