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Topographical features of the membrane of Poterioochromonas malhamensis after colchicine and osmotic treatment

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Abstract

Changes in membrane topography in the flagellate Poterioochromonas malhamensis, as a result of colchicine and osmotic-stress treatments, have been studied using freeze-fracturing and thin sectioning. Ridges, but not rows of intramembrane particles, in the PF-face which denote the position of underlying cortical microtubules, together with the ridge associated with their point of origin (flagellar root fibre 1), dissappear after colchicine or short-term (5 min) osmotic treatments. Cortical microtubules are destroyed as a result of the former, but not the latter treatment. Longer periods in osmoticum allow a recovery of the microtubule — associated membrane ridges. Despite careful isosmotic fixations distinct cross-bridges between microtubules and the plasmalemma were not discernible in thin section.

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References

  • Bouck, G.B., Brown, D.L.: Microtubule biogenesis and cell shape in Ochromonas. I. The distribution of cytoplasmic and mitotic microtubules. J. Cell Biol. 56, 340–359 (1973)

    Article  PubMed  Google Scholar 

  • Branton, D., Bullivant, S., Gilula, N.B., Karnovsky, M.J., Moor, H., Mühlethaler, K., Northcote, D.H., Packer, L., Satir, B., Satir, P., Speth, V., Staehelin, L.A., Steere, R.L., Weinstein, R.S.: Freeze-etching nomenclature. Science 190, 54–56 (1975)

    PubMed  Google Scholar 

  • Brown, D.L., Bouck, G.B.: Microtubule biogenesis and cell shape in Ochromonas. II. The role of nucleating sites in shape development. J. Cell Biol. 56, 360–378 (1973)

    Article  PubMed  Google Scholar 

  • Brown, D.L., Bouck, G.B.: Microtubule biogenesis and cell shape in Ochromonas. III. Effects of the herbicidal inhibitor isopropyl-N phenylcarbamate on shape and flagellar regeneration. J. Cell Biol. 61, 514–536 (1974)

    Article  PubMed  Google Scholar 

  • Heath, I.B.: A unfied hypothesis for the role of membrane bound enzyme complexes and microtubules in plant cell wall synthesis. J. Theor. Biol. 48, 445–449 (1974)

    PubMed  Google Scholar 

  • Hepler, P.K., Palevitz, B.A.: Microtubules and microfilaments. Annu. Rev. Plant Physiol. 25, 309–362 (1974)

    Article  Google Scholar 

  • Herth, W.: Details der Mikrotubulus-Anordnung und der Mikrotubulus-Mikrofilament-Membran-Beziehung im cytoplasmatischen Fortsatz des Chrysoflagellaten Poterioochromonas. Cytobiologie 18, 185–186 (1978)

    Google Scholar 

  • Hunt, R.C., Ellar, D.J.: Isolation of the plasma membrane of a trypanosomatid flagellate: general characterization and lipid composition. Biochim. Biophys. Acta 339, 173–189 (1974)

    Google Scholar 

  • Kauss, H.: Osmotic regulation in algae. Progr. in Phytochemistry 5, 1–27 Oxford and New York, Pergamon Press: 1979

    Google Scholar 

  • Ledbetter, M.C., Porter, K.R.: A microtubule in plant cell fine structure. J. Cell. Biol. 19, 239–250 (1963)

    Article  Google Scholar 

  • Linder, J.S., Staehelin, L.A.: Plasma membrane specializations in a trypanosomatid flagellate. J. Ultrastruct. Res. 60, 246–262 (1977)

    PubMed  Google Scholar 

  • Marchant, H.J.: Microtubules associated with the plasma membrane isolated from protoplast of the green alga Mougeotia. Exp. Cell Res. 115, 25–30 (1978)

    PubMed  Google Scholar 

  • Marchant, H.J., Hines, E.R.: The role of microtubules and cell wall deposition in elongation of regenerating protoplasts in Mougeotia. Planta 146, 41–48 (1979)

    Google Scholar 

  • Schnepf, E., Röderer, G., Herth, W.: The formation of the fibrils in the lorica of Poterioochromonas stipitata: tip growth, kinetics, site, orientation. Planta 125, 45–62 (1975)

    Google Scholar 

  • Schnepf, E., Deichgräber, G., Röderer, G., Herth, W.: The flagellar root apparatus, the microtubular system and associated organelles in the chrysophycean flagellate Poterioochromonas malhamensis Peterfi (syn. Poterioochromonas stipitata Scherffel and Ochromonas malhamensis Pringsheim). Protoplasma 92, 87–107 (1977)

    Google Scholar 

  • Schobert, B., Untner, E., Kauss, H.: Isofloridisid und die Osmoregulation bei Ochromonas malhamensis. Z. Pflanzenphysiol. 67, 385–398 (1972)

    Google Scholar 

  • Wessel, D., Robinson, D.G.: Studies on the contractile vacuole of Poterioochromonas malhamensis Peterfi. I. The structure of the alveolate vesicles. Eur. J. Cell Biol. 19, 60–66 (1979)

    Google Scholar 

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  1. Abteilung Cytologie des Pflanzenphysiologischen Instituts, Universität Göttingen, Untere Karspüle 2, D-3400, Göttingen, Federal Republic of Germany

    D. G. Robinson & H. Quader

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  1. D. G. Robinson
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  2. H. Quader
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Robinson, D.G., Quader, H. Topographical features of the membrane of Poterioochromonas malhamensis after colchicine and osmotic treatment. Planta 148, 84–88 (1980). https://doi.org/10.1007/BF00385446

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  • DOI: https://doi.org/10.1007/BF00385446

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