Abstract
We studied the modulation of the number of membrane-bound naphthaleneacetic acid (NAA)-binding sites during the growth cycle of tobacco cells in batch culture. Both cell number and specific NAA-binding increased exponentially, but at different rates and for different periods. This caused a characteristic modulation of the number of binding sites per cell during the growth cycle: During the first day of the lag phase this number decreased; in the exponential phase it rose markedly, and in the stationary phase it was constant.
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Abbreviations
- MES:
-
4-morpholinoethanesulphonic acid
- NAA:
-
1-naphthaleneacetic acid
References
Batt, S., Wilkins, M.B., Venis, M.A. (1976) Auxin-binding to corn coleoptile membranes: kinetics and specificity. Planta 130, 7–13
Bogers, R.J., Kulescha, Z., Quint, A., van Vliet, Th. B., Libbenga, K.R. (1980) The presence of a soluble auxin receptor and the metabolism of 3-indoleacetic acid in tobacco-pith exlants. Plant Sci. Lett. 19, 311–317
Dohrmann, U., Hertel, R., Kowalik, H. (1978) Properties of auxin-binding sites in different subcellular fractions from maize coleoptiles. Planta 140, 97–106
Döllstädt, R., Hirschberg, K., Winkler, E., Hübner, G. (1976) Bindung von Indolylessigsäure und Phenoxyessigsäure an Fraktionen aus Epikotylen und Wurzeln von Pisum sativum L. Planta 130, 105–111
Henshaw, G.G., Jha, K.K., Mehta, A.R., Shakeshaft, D.J., Street, H.E. (1966) Studies on the growth in culture of plant cells I. Growth patterns in batch-propagated suspension cultures. J. Exp. Bot. 17, 362–377
Hertel, R., Thompson, K.S., Russo, V.E.A. (1972) In-vitro auxin-binding to particulate cell fractions from corn coleoptiles. Planta 107, 325–340
King, P.J., Mansfield, K.J., Street, H.E. (1973) Control of growth and cell division in plant cell suspension cultures. Can. J. Bot. 51, 1807–1823
King, P.J., Street, H.E. (1977) Growth patterns in cell cultures. In: Plant tissue and cell culture, pp. 307–388, Street, H.E., ed. Blackwell Scientific Publications, Oxford London Edinburgh Melbourne.
Linsmaier, E.M., Skoog, F. (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18, 100–127
Nash, D.T., Davies, M.E. (1972) Some aspects of growth and metabolism of Paul's scarlet rose cell suspensions. J. Exp. Bot. 23, 75–91
Nato, A., Mathieu, Y. (1978) Changes in phosphoenolpyruvate carboxylase and ribulose-biphosphate carboxylase activities during the photoheterotrophic growth of Nicotiana tabacum (cv Xanthi) cell suspensions. Plant Sci. Lett. 13, 49–56
Normand, G., hartmann, M.A., Schuber, F., Benveniste, P. (1975) Caractérisation de membranes de coléoptiles de maïs fixant l'auxine et l'acide N-naphtyl phtalamique. Physiol. Vég. 13, 743–761
Oostrom, H. (1980) Investigations on a soluble auxin receptor. Thesis, Leiden
Oostrom, H., van Loopik-Detmers, M.A., Libbenga, K.R. (1975) A high-affinity receptor for indoleacetic acid in cultured tobacco pith explants. FEBS Lett. 59, 194–197
Oostrom, H., Kulescha, Z., van Vliet, Th.B., Libbenga, K.R. (1980) Characterization of a cytoplasmic auxin receptor from tobacco pith callus. Planta 149, 44–47
Ray, P.M., Dohrmann, U., Hertel, R. (1977) Specificity of auxinbinding sites on maize coleoptile membranes as possible receptor sites for auxin action. Plant Physiol. 60, 585–591
Vreugdenhil, D., Burgers, A., Libbenga, K.R. (1979) A particlebound auxin receptor from tobacco pith callus. Plant Sci. Lett. 16, 115–121
Vreugdenhil, D., Harkes, P.A.A., Libbenga, K.R. (1980) Auxinbinding by particulate fractions from tobacco leaf protoplasts. Planta 150, 9–12
Wardrop, A.J., Polya, G.M. (1980) Co-purification of pea and bean leaf soluble auxin-binding proteins with ribulose-1,5-biphosphate carboxylase. Plant Physiol. 66, 105–111
Wilson, G., Marron, P. (1978) Growth and anthraquinone biosynthesis by Galium mollugo L. cells in batch culture and chemostat culture. J. Exp. Bot. 29, 837–851
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Vreugdenhil, D., Burgers, A., Harkes, P.A.A. et al. Modulation of the number of membrane-bound auxin-binding sites during the growth of batch-cultured tobacco cells. Planta 152, 415–419 (1981). https://doi.org/10.1007/BF00385357
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DOI: https://doi.org/10.1007/BF00385357