Summary
Substrate levels of sucrose were shown to reduce chlorophyll synthesis in carrot tissue culture strain CRT1 but not in strain CRT2. In CRT1 the effect was shown to be a suppression of greening specifically by sucrose rather than a reducing sugar requirement for chlorophyll synthesis. In CRT1 sucrose caused both a reduction in chloroplast numbers per cell and a suppression of lamellar development in plastids. This effect on chloroplast structure was consistent with the observed reduced photosynthetic efficiency (micromoles CO2 per hour per mg chlorophyll) of CRT1 calluses grown on sucrose.
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App, A. A., Jagendorf, A. T.: Repression of chloroplast development in Euglena gracilis by substrates. Protozoology 10, 340–343 (1963).
Beiderbeck, R., Nitsche, H.: The chlorophyll content of tumors from pale and green leaf tissues of Kalanchoe Fedtschenkoi. Planta (Berl.) 92, 57–63 (1970).
Duranton, H., Schantz, R., Kientz, J.-G.: Influence des glucides sur la synthèse de la chlorophylle chez les explants calibrés de Topinambour cultivés in vitro. Bull. Soc. franç. Physiol. végét. 10, 186–194 (1964).
French, C. S.: Encyclopaedia of plant physiology, vol. 5, no. 1, p. 279, ed. W. Ruhland. Berlin-Göttingen-Heidelberg: Springer 1960.
Hanson, A. D., Edelman, J.: Phloem in carrot calluses. Planta (Berl.) 93, 171–174 (1970).
——: Secretion of photosynthetic products by carrot tissue cultures. Planta (Berl.) 98, 97–108 (1971).
Posner, H. B.: Inhibitory effect of carbohydrate on flowering in Lemna perpusilla. Plant Physiol. 45, 687–690 (1970).
Schoolar, A. L., Edelman, J.: Production and secretion of sucrose by sugar-cane leaf tissue. J. exp. Bot. 21, 49–57 (1970).
Shihira-Ishikawa, I., Hase, E.: Nutritional control of cell pigmentation in Chlorella protothecoides with special reference to the degeneration of chloroplast induced by glucose. Plant and Cell Physiol. 5, 227–240 (1964).
Wintermans, J. G. F. M., DeMots, A.: Spectrophotometric characterizations of chlorophylls a and b and their pheophytins in ethanol. Biochem. biophys. Acta (Amst.) 109, 448–453 (1965).
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Edelman, J., Hanson, A.D. Sucrose suppression of chlorophyll synthesis in carrot callus cultures. Planta 98, 150–156 (1971). https://doi.org/10.1007/BF00385347
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DOI: https://doi.org/10.1007/BF00385347