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Untersuchungen zur Beziehung zwischen der lichtabhängigen und der redoxabhängigen Änderung des Membranpotentials bei Griffithsia setacea

Investigations concerning the correlation between the light-dependent and the redox-dependent change of the membrane potential in Griffithsia setacea

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Summary

  1. 1.

    With the red alga Griffithsia setacea in the dark the addition of reduced DCPIP effects a depolarization of the membrane potential. The kinetics of this redox-dependent depolarization is in good conformity with that of the light-dependent depolarization. Ascorbate as well as cystein are efficient as reducing substance.

  2. 2.

    By simultaneous application of light and reduced DCPIP it can be demonstrated that the light-dependent and the redox-dependent change of the membrane potential may be involved in the same system.

  3. 3.

    The redox-dependent membrane potential change is a function of the redox potential difference between oxidized and reduced DCPIP. In the linear range of this relation the simultaneously measured light-dependent membrane potential change corresponds to a redox potential difference of 124mV.

  4. 4.

    The redox-dependent membrane potential change, like the light-dependent membrane potential change, is dependent on the pH of the medium. Between pH 9.0 and pH 9.5a transition from a depolarization to a hyperpolarization of the membrane potential can be observed.

  5. 5.

    The results support the hypothesis that the redox level of a component in the photosynthetic electron transport chain may steer the light-dependent and the redox-dependent change of the membrane potential.

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Authors and Affiliations

  1. Botanisches Institut der Universität Marburg, Pilgrimstein 4, D-3550, Marburg, Bundesrepublik Deutschland

    G. Throm

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  1. G. Throm
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Einige Fakten dieser Arbeit wurden auf dem Internationalen Photobiologie-Kongreß, Bochum (1972), vorgetragen.

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Throm, G. Untersuchungen zur Beziehung zwischen der lichtabhängigen und der redoxabhängigen Änderung des Membranpotentials bei Griffithsia setacea . Planta 112, 273–284 (1973). https://doi.org/10.1007/BF00385331

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  • DOI: https://doi.org/10.1007/BF00385331

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