Summary
After local assimilation of NaH14CO3 by an old frond of Laminaria hyperborea, radioactive photosynthate is translocated to the growing region of the thallus. The pathway of this long-distance transport was studied by histoautoradiography. Cellular localization of the conducting channels was accomplished by new autoradiographic methods including freeze-substitution and 1 μm-cuttings of epoxy resin embedded tissue. In the autoradiographs, patches of silver grains overlying single trumpet-filaments (=Trompetenzellen) indicated that downward translocation occurs in these cells only. It could be shown that predominantly young trumpetfilaments contain the bulk of labeling. It is concluded that the young filaments rather than the older ones are particularly active in translocation. A lateral movement of labelled material was not observed except in the growing region.
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Literatur
Benditt, E.P., Martin, G.M., Platter, H.: Application of freeze-drying and formaldehyde-vaporfixation to radioautographic localization of soluble amino acids. Symposia of the Internat. Soc. for Cell Biol., vol. 4, p. 75, eds. C.P. Leblond, K.B. Warren, New York: Academic Press 1965.
Branton, D., Jacobson, L.: Dry, high resolution autoradiography. Stain Technol. 37, 239–242 (1962).
Feder, N., O'Brien, T.P.: Plant microtechnique: some principles and new methods. Amer. J. Bot. 55, 123–142 (1968).
Fritz, E., Eschrich, W.: 14C-Mikroautoradiographie wasserlöslicher Substanzen im Phloem. Planta (Berl.) 92, 267–281 (1970).
Kilian, K.: Beitrage zur Kenntnis der Laminarien. Z. Bot. 3, 433–494 (1911).
Morgenstern, E.: Vergleichende lichtoptische Untersuchungen im Rahmen elektronenmikroskopischer Arbeiten an ultradünne Schnitten. II. Färbemethoden. Mikroskopie 25, 250–260 (1969).
Nicholson, N.L., Briggs, W.R.: Translocation of photosynthate in the brown alga Nereocystis. Amer. J. Bot. 59, 97–106 (1972).
Oliver, F.W.: On the obliteration of the sieve-tubes in Laminariaceae. Ann. Bot. 1, 95–117 (1887).
Parker, B.C.: Translocation in the giant kelp Macrocystis I. Rates, direction, quantity of 14C-labeled products and fluorescein. J. Phycol. 1, 41–46 (1965).
Parker, B.C.: Translocation in Macrocystis. III. Composition of sieve tubes exudate and identification of the major 14C-labeled products. J. Phycol. 2, 38–41 (1966).
Schmitz, K., Lüning, K., Willenbrink, J.: CO2-Fixierung und Stofftransport in benthischen marinen Algen. II. Zum Ferntransport 14C-markierter Assimilate bei Laminaria hyperborea und Laminaria saccharina. Z. Pflanzenphysiol. 67, 418–429 (1972).
Schmitz, K., Willenbrink, J.: Histoautoradiographischer Nachweis 14C-markierter Assimilate im Phloem. Z. Pflanzenphysiol. 58, 97–107 (1967).
Schnepf, E.: Golgi-Apparat und Sekretbildung in den Drüsenzellen der Schleimgänge von Laminaria hyperborea. Naturwissenschaften 50, 674 (1963).
Sykes, M.G.: Anatomy and histology of Macrocystis pyrifera and Laminaria saccharina. Ann. Bot. 22, 291–325 (1908).
Ziegler, H., Ruck, I.: Untersuchungen über die Feinstruktur des Phloems. III. Mitteilung Die “Trompetenzellen” von Laminaria-Arten. Planta (Berl.) 73, 62–73 (1967).
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Steinbiß, HH., Schmitz, K. CO2-Fixierung und Stofftransport in benthischen marinen Algen. Planta 112, 253–263 (1973). https://doi.org/10.1007/BF00385329
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DOI: https://doi.org/10.1007/BF00385329