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Metabolism of [2-14C] acetate to amino acids and proteins in segments of bean seedling roots

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Summary

14C from [2-14C] acetate was found to be incorporated into soluble and protein amino acids in substantial amounts by bean root apices. The 14C was spread through a wide range of amino acids in both these fractions. Glutamic acid was found to be heavily labelled with 14C in both soluble and protein amino acid fractions. The data are discussed in relation to present ideas on transport and utilization of amino acids in root systems.

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References

  • Clowes, F. A. L.: Protein synthesis in root meristens. J. exp. Bot. 9, 229–234 (1958).

    Google Scholar 

  • Clowes, F. A. L.: Nutrition and the quiescent centre of root meristems. Planta (Berl.) 90, 340–348 (1970).

    Google Scholar 

  • Clowes, F. A. L., Juniper, B. E.: Plant cells. Oxford: Blackwell 1968).

    Google Scholar 

  • Fowler, M. W., ap Rees, T.: Carbohydrate oxidation during differentiation of roots of Pisum sativum. Biochem. biophys. Acta (Amst.) 201, 33–44 (1970).

    Google Scholar 

  • Fowler, M. W., ap Rees, T.: Metabolism of glucose to amino acids in the apex of roots of Pisum sativum. Biochim. biophys. Acta (Amst.) 304, 326–331 (1973).

    Google Scholar 

  • Harley, J. L., Beevers, H.: Acetate utilisation in maize roots. Plant Physiol. 38, 117–123 (1963).

    Google Scholar 

  • Ingle, J., Beevers, L., Hageman, R. H.: Metabolic changes associated with the germination of corn. I. Changes in weight and metabolites and their redistribution in the embryo-axis, scutellum and endosperm. Plant Physiol. 39, 735–740 (1964).

    Google Scholar 

  • Ingversen, J., Ivanko, S.: Investigations on the assimilation of nitrogen by maize roots and the transport of some major nitrogen compounds by xylem sap. II. Incorporation of taken-up nitrogen into free amino acids and protein of maize roots. Physiol. Plantarum (CpH.) 24, 199–204 (1971).

    Google Scholar 

  • Ivanko, S., Ingversen, J.: Investigations on the assimilation of nitrogen by maize roots and the transport of some major nitrogen compounds by xylem sap. I. Nitrate and ammonia uptake and assimilation in the major nitrogen fractions of nitrogen-starved maize roots. Physiol. Plantarum (CpH.) 24, 59–65 (1971).

    Google Scholar 

  • Jensen, W. A.: A morphological and biochemical analysis of the early phases of cellular growth in the root tip if Vicia faba. Exp. Cell Res. 8, 506–522 (1955).

    PubMed  Google Scholar 

  • Jensen, W. A.: The incorporation of [14C] adenine and [14C] phenylalanine by developing root tip cells. Proc. nat. Acad. Sci. (Wash.) 34, 1038–1046 (1957).

    Google Scholar 

  • Loening, U. E.: Changes in the microsomal components accompanying cell differentiation of pea-seedling roots. Biochem. J. 81, 254–260 (1961).

    PubMed  Google Scholar 

  • MacLennan, D. H., Beevers, H., Harley, J. L.: Compartmentation of acids in plant tissues. Biochem. J. 89, 316–327 (1963).

    Google Scholar 

  • Morgan, C., Reith, W. S.: Composition and quantitative relations of protein and related fractions in developing root cells. J. exp. Bot. 5, 119–135 (1954).

    Google Scholar 

  • Oaks, A.: The source of amino acids for protein synthesis in maize root tips. Plant Physiol. 37, ix (1962).

    Google Scholar 

  • Oaks, A.: The soluble leucine pool in maize root tips. Plant Physiol. 40, 142–149 (1965a).

    PubMed  Google Scholar 

  • Oaks, A.: The effect of leucine on the biosynthesis of leucine in maize root tips. Plant Physiol. 40, 149–155 (1965b).

    PubMed  Google Scholar 

  • Oaks, A.: Transport of amino acids to the maize root. Plant Physiol. 41, 173–180 (1966).

    Google Scholar 

  • Oaks, A.: Synthesis of macromolecules in maize root tips. Canad. J. Bot. 45, 385–394 (1967).

    Google Scholar 

  • Oaks, A., Beevers, H.: The requirement for organic nitrogen in Zea mays embryos. Plant Physiol. 39, 37–43 (1964).

    Google Scholar 

  • Oaks, A., Johnson, F. J.: Effect of sugars on amino acid biosynthesis in maize root tips. Canad. J. Bot. 48, 117–124 (1970).

    Google Scholar 

  • Pate, J. S.: Physiological aspects of inorganic and intermediate nitrogen metabolism (with special reference to the legume Pisum arvense L.). In: Recent aspects of nitrogen metabolism in plants, pp. 219–240 Hewitt, E. J., Cutting, C. V., ed. London: Academic Press 1968.

    Google Scholar 

  • Siekevitz, P.: Uptake of radioactive alanine in vitro into the proteins of rat liver fractions. J. biol. Chem. 195, 549–565 (1952).

    PubMed  Google Scholar 

  • Street, H. E.: The physiology of root growth. Ann. Rev. Plant Physiol. 17, 315–344 (1966).

    Google Scholar 

  • Turner, J. C.: Triton X-100 scintillant for carbon 14 labelled materials. Int. J. appl. Radiat. Isotopes 19, 557–563 (1968).

    Google Scholar 

  • Wong, W. J. L., ap Rees, T.: Carbohydrate oxidation in stele and cortex isolated from roots of Pisum sativum. Biochim. biophys. Acta (Amst.) 252, 296–304 (1971)

    Google Scholar 

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Fowler, M.W. Metabolism of [2-14C] acetate to amino acids and proteins in segments of bean seedling roots. Planta 112, 235–242 (1973). https://doi.org/10.1007/BF00385327

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  • DOI: https://doi.org/10.1007/BF00385327

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