Summary
After pulse-labelling with [32P]orthophosphate of higher plants (Lemna perpusilla (Torrey) and Lemna gibba (L.)) which were previously grown in a P-free medium for 24 h, the [32P]-radioactivity is partially incorporated into condensed inorganic phosphates. The isolation of the latter was achieved by joint extraction with the nucleic acids and fractionation on methylated serum albumin and Kieselgur (MAK). Two-dimensional thin layer chromatography on a mixture of cellulose and microcrystalline cellulose revealed that 32P-radioactivity was present in linear oligophosphates with 2 to 7 residues as well as in cyclic tri-, tetra-, penta- and hexametaphosphates. Among the low-molecular condensed inorganic phosphates the trimetaphosphate contained significantly more radioactivity than the other compounds. It is supposeded that the condensed phosphates are involved in the regulation of the ATP-ADP-Pi-system.
Literatur
Agrawal, M., Krishnan, P. S.: An unspecific metaphosphatase from the leaves of the topioca plant (Manihot utilissima). Enzymologia 21, 18–22 (1959)
Eigener, U., Bock, E.: Aut- und Abbau der Polyphosphatfraktion in Zellen von Nitrobacter winogradskyi Buch. Mikrobiol. 81, 367–378 (1972)
Harold, F. M.: Inorganic polyphosphates in biology: Structure, metabolism and function. Bact. Rev. 30, 772–794 (1966)
Klein, R. M.: Nitrogen and phosphorus fractions, respiration and structure of normal and crown gall tissues of tomato. Plant Physiol. 27, 335–354 (1952)
Kuhl, A.: Inorganic phosphorus uptake and metabolism. In: Physiology and biochemistry of algae, ed. R. A. Lewin, p. 211–299. New York: Academic Press 1962
Miyachi, S.: Inorganic polyphosphate in spinach leaves. J. Biochem. 50, 367–371 (1961)
Niemeyer, R.: Isolierung und Reinigung der Poly- und Metaphosphate aus der Blaualge Anacystis nidulans. Diss. Tübingen (1969)
Niemeyer, R., Richter, G.: Schnellmarkierte Polyphosphate und Metaphosphate bei der Blaualge Anacystis nidulans. Arch. Mikrobiol. 69, 54–59 (1969)
Niemeyer, R., Richter, G.: Rapidly labelled polyphosphates in acetabularia. In: Biology and radiobiology of anucleate systems II. Plant cells. Ed. S. Bonotto, R. Goutier, R. Kirchmann and J.-R. Maisin, p. 225–236. New York: Academic Press 1972
Pirson, A., Seidel: Zell- und Stoffwechselphysiologische Untersuchungen an der Wurzel von Lemna minor L. unter besonderer Berücksichtigung von Kalium- und Calziummangel. Planta (Berl.) 38, 431 (1950)
Pierpoint, W. S.: The phosphatases and meta-phosphatase activities in pea extracts. Biochem. J. 65, 67–76 (1957a)
Pierpoint, W. S.: The phosphoesterase of pea plants (Pisum sativum L.). Biochem. J. 67, 466–472 (1957b)
Rothenbach, E. F., Hinkelmann, St.: Über Poly- und Metaphosphatasen der Gerste. Naturwissenschaften 41, 555 (1954)
Tewari, K. K., Singh, M.: Acid soluble and acid insoluble inorganic polyphosphates in Cuscuta reflexa. Phytochemistry 3, 341–347 (1964)
Vagabov, U. M., Kulaev, J. S.: Inorganic polyphosphates in corn roots. Dokl. Akad. Nauk. SSSR 158, 218–220 (1964)
Weinberg, R., Orton, W. L.: Synthesis and breakdown of the polyphosphate fraction and acid phosphomonoesterase of Saccharomyces mellis and their location in the cell. J. Bact. 89, 746–747 (1965)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Niemeyer, R. Poly- und Metaphosphate in höheren Pflanzen (Lemnaceae). Planta 122, 303–305 (1975). https://doi.org/10.1007/BF00385280
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00385280