Skip to main content
Log in

Staining of sieve tube mitochondria in coleoptiles of rice (Oryza sativa L.) with diaminobenzidine

  • Published:
Planta Aims and scope Submit manuscript

Summary

Diaminobenzidine has been used to locate sites of oxidizing cytochromes in rice coleoptiles. The mitochondria of mature sieve tubes show a positive reaction which to visual inspection appears as strong as the reaction of companion cell mitochondria.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Abbreviations

DAB:

diaminobenzidine

References

  • Crafts, A. S., Crisp, C. E.: Phloem transport in plants. San Francisco: Freeman 1971

    Google Scholar 

  • Cronshaw, J.: Phloem differentiation and development. In: Dynamic aspects of plant ultrastructure, p. 391–413, Robards, A. W., ed. London-New York: MeGraw-Hill 1974

    Google Scholar 

  • Öpik, H.: Effect of anaerobiosis on respiratory rate, cytochrome oxidase activity and mitochondrial structures in coleoptiles of rice (Oryza sativa L.). J. Cell Sci. 12, 725–739 (1973)

    Google Scholar 

  • Öpik, H.: The reaction of mitochondria in the coleoptiles of rice (Oryza sativa L.) with diaminobenzidine. J. Cell Sci., in press (1974)

  • Reith, A., Schüler, B.: Demonstration of cytochrome oxidase activity with diaminobenzidine. A biochemical and electron microscope study. J. Histochem. Cytochem. 20, 583–589 (1972)

    Google Scholar 

  • Seligman, A. M., Karnovsky, M. J., Wasserkrug, H., Hanker, J. S.: Nondroplet ultrastructural demonstration of cytochrome oxidase activity with a polymerizing osmiophilic reagent, diaminobenzidine (DAB). J. Cell Biol. 38, 1–14 (1968)

    Google Scholar 

  • Venable, J. H., Coggeshall, R.: A simplified lead citrate stain for use in electron microscopy. J. Cell Biol. 25, 407–408 (1965)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Öpik, H. Staining of sieve tube mitochondria in coleoptiles of rice (Oryza sativa L.) with diaminobenzidine. Planta 122, 269–271 (1975). https://doi.org/10.1007/BF00385275

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00385275

Keywords

Navigation